HPLC测定乐脉片中丹酚酸B的含量

Determination of Salvianolic Acid B in Lemai Tablets by HPLC

摘要:

目的: 建立HPLC测定乐脉片中丹酚酸B含量的方法。 方法: 采用Waters SymmetryShield RP18色谱柱(3.9 mm×150 mm,5 μm),流动相乙腈-甲醇-1.7%甲酸水溶液(4∶27∶69),检测波长286 nm。 结果: 丹酚酸B在0.20~2.5 μg成良好的线性关系(r=0.999 9),平均回收率为100.2%(n=6,RSD 2.0%)。 结论: 方法简便、快速准确、灵敏度高,重复性好,可作为乐脉片中丹酚酸B的含量测定。

Abstract:

Objective:To establish a method for determination of salvianolic acid B in Lemai tablets by HPLC. Method: A Waters SymmetryShield RP18 column (3.9 mm×150 mm,5 μm) was used with the mobile phase of acetonitrile-methnol-1.7% formic acid (4∶27∶69) at the detection wavelength of 286 nm. Result: The calibration curve was linear in the range of 0.20-2.5 μg (r=0.999 9). The average recovery was 100.2% (n=6, RSD 2.0%). Conclusion: The method is convenient, quick, accurate and suitable for the determination of salvianolic acid B in Lemai tablets.