狗脊中化学成分及其对DPPH清除作用研究

Chemical Constituents from Cibotium barometz and their Scavenging Activities for DPPH Radical

摘要:

目的: 研究狗脊的化学成分,探讨其对二苯代苦味基肼自由基(DPPH)的清除作用。 方法: 采用硅胶色谱、凝胶色谱、高效液相色谱等技术对狗脊70%乙醇提取物进行分离纯化,通过波谱方法鉴定其结构,并对化合物进行清除DPPH活性试验。 结果: 从狗脊70%乙醇提取物中分离得到10个成分,分别为1-O-咖啡酰-β-D-葡萄糖( 1 ),6-O-咖啡酰-D-葡萄糖( 2 ),3-O-咖啡酰-D-葡萄糖( 3 ),3-hydroxymethyl-2(5H)-furanone( 4 ),β-miroside( 5 ),cibotiumbaroside A( 6 ),原儿茶酸( 7 ),甘露糖( 8 ),corchoionoside C( 9 ),曲酸( 10 )。对DPPH清除能力试验结果表明,3个化合物对DPPH自由基都有较强的清除能力。 结论: 化合物 1 ~ 5 均为从狗脊中首次分离得到。化合物 1 , 3 , 6 均具有较强的抗氧化活性,其中化合物1的清除效果与维生素C(Vit C)相当。

Abstract:

Objective:Study on the chemical constituents from Cibotium barometz and their scavenging activities for 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical. Method: The chemical constituent from70% alcohol extract of C. barometz was isolated on chromatograph of silica gel and sephadex LH-20 and HPLC, their structure were elucidated on the basis of spectra data and the compounds were tested for their scavenging activities on DPPH radical. Result: Ten compounds were purified and their structure were identified as 1-O-caffeyl-D-glucopyranose ( 1 ), 6-O-caffeyl-D-glucopyranose ( 2 ), 3-O-caffeyl-D-glucopyranose ( 3 ), 3-hydroxymethyl-2(5H)-furanone ( 4 ), β-miroside( 5 ), cibotiumbaroside A( 6 ), protocatechuic acid ( 7 ), glucose ( 8 ), mannose ( 9 ), Corchoionoside C ( 10 ), kojic acid ( 11 ). Three isolated compounds showed strong scavenging activities on DPPH radical. Conclusion: Compound 1-5 were isolated for the first time from C. barometz. among of them, compound 1 , compound 3 and compound 6 showed significant antioxidant activity, and the scavenging activity of compound 1 was similar to that of Vit C.