不同基源郁金挥发油GC指纹图谱的比较

Comparison Study on GC Fingerprints of Curcumae Radix Volatile Oil

摘要:

目的: 通过建立4种基源郁金挥发油的指纹图谱,对其指纹图谱进行比较研究,为制定郁金药材合理的质量控制标准提供参考。 方法: 采用气相色谱法,HP-5(0.32 μm×0.32 mm×30 m)柱;氢火焰离子化检测器(FID);进样口温度280 ℃,检测器温度300 ℃;程序升温初温125 ℃,保持5 min;速率3 ℃·min^-1,升至170 ℃,保持5 min;速率9 ℃·min^-1,升至260 ℃,保持0 min。 结果: 对4种基源20批郁金挥发油进行分析,分别建立了4种郁金挥发油的指纹图谱。黄丝郁金和温郁金样品的相似度均在0.95以上;绿丝郁金样品相似度均在0.99以上;桂郁金相似度在0.9左右。 结论: 不同基源郁金挥发油的化学组成差异较大,各自对照图谱能反映其特征,为科学制定郁金药材的质量标准提供了参考。

Abstract:

Objective:To provide a reference of reasonable quality control standards through a comparative study of the four fingerprints of four origin plants of Curcumae Radix. Method: The separation was performed on HP-5 0.32 μm×0.32 mm×30 m column,hydrogen flame ionization detector (FID); injector temperature was 280 ℃, detector temperature was 300 ℃; the column oven was begun at 125 ℃,then programmed from 125 ℃ to 170 ℃ at 3 ℃·min^-1, then programmed from 170 ℃ to 260 ℃ at 9 ℃·min^-1 and final, held 0 min. Result: To analyse 20 batches of volatile oil, the fingerprints of four origin plants of Curcumae Radix were established. The fingerprint similarity value of the Curcuma longa and C.wenyujin is above 0.95; the fingerprint similarity value of the C.phaeocaulis is above 0.99; the fingerprint similarity value of the C.kwangsiensis is about 0.90. Conclusion: It displayed marked differences between four origin plants of Curcumae Radix volatile oil chemical constituents and everyone's fingerprint could describe own chemical constituents. So we provide the data support for establishing scientific quality control standards of Curcumae Radix.