五子衍宗方对实验性隐睾小鼠生精功能的保护作用

Protective Effects of Wuzi Yanzong Decoction on Experimental Cryptorchid Mice

摘要:

目的: 研究五子衍宗方对实验性隐睾小鼠生精功能的保护作用。 方法: 取5~6周龄BALB/c小鼠,手术建立小鼠隐睾模型,分为假手术组,模型组,五子衍宗方低、高剂量(100,200 mg·kg^-1)组,手术14 d后给药,用药14 d,于末次给药24 h 后检测小鼠的睾丸质量、精子密度、活率,光、电镜观察睾丸组织学结构的变化,检测小鼠睾丸内谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化。 结果: 五子衍宗方能够显著增加模型小鼠的睾丸质量(P＜0.01),明显增加精子密度(P＜0.05),提高精子活率(P＜0.01),改善隐睾小鼠睾丸组织的病理性变化,显著升高SOD和GSH-Px活性(P＜0.05或P＜0.01),显著降低MDA含量(P＜0.01)。 结论: 五子衍宗方提高睾丸组织内抗氧化损伤能力,从而减轻隐睾对小鼠睾丸生殖功能的损害。

Abstract:

Objective:To investigate the protective effect of Wuzi Yanzong decoction on experimental cryptorchidism mice. Method: Five-six weeks old BALB/c mice cryptorchidism was induced and divied into four groups as sham, model, low dose of Wuzi Yanzong decoction (100 mg·kg^-1), high dose of Wuzi Yanzong decoction (200 mg·kg^-1). At the end of the study, testis tissues were collected for detection of testes weight, sperm density,live rate, histological structure changes of testis, detection intestes glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) activity and the malonyldialdehyde(MDA) content changes. Result: The Wuzi Yanzong decoction treated mice in the cryptorchid group significantly increased the mice testis weight (P＜0.01), sperm density (P＜0.05), sperm live rate (P＜0.01) and showed an improved histologic appearance. Wuzi Yanzong decoction treatment significantly increased the reduced SOD and GSH-Px enzyme activities (P＜0.05 or P＜0.01) and decreased the elevated tissue malondialdehyde (MDA) levels (P＜0.01) in testis tissues samples. Conclusion: Wuzi Yanzong decoction is a potential beneficial agent to reduce testicular damage in cryptorchid mice by decreasing oxidative stress.