益气活血方对放射性肺损伤大鼠内皮素-1, γ-干扰素表达的影响

Effect of Yiqi Huoxue Recipe on expression of ET-1/γ-IFN in Rats with Radiation-induced Lung Injury

摘要:

目的: 观察益气活血方对放射性肺损伤大鼠血清及肺组织内皮素-1(ET-1)、γ-干扰素(γ-IFN)表达的影响。 方法: 将66只Wistar大鼠随机分为照射加益气活血方组(益气活血方组)30只、单纯照射组(模型组)30只、正常对照组(对照组)6只。于受照当天开始,益气活血方组每天予益气活血中药10 g·kg^-1,ig,模型组及对照组予蒸馏水10 g·kg^-1,ig,直至动物被处死取材。益气活血方组与模型组均于首次照射后第4,6,8,12,26周末各取6只,对照组6只于第8周末处死,均取血清及右肺组织,检测ET-1,γ-IFN的表达。 结果: 益气活血方组及模型组ET-1的肺组织表达及血清值均于照射后第4周升高,8周达到高峰,26周下降,益气活血方组各时相ET-1的表达肺组织及血清值均低于模型组(P<0.05)。而γ-IFN值于第4周开始降低,12周至低谷,26周回升。益气活血方组γ-IFN的表达肺组织及血清值均高于模型组(P<0.05)。 结论: 益气活血方能调控ET-1,γ-IFN的表达及分泌,对放射性肺损伤具有较好的防治作用。

Abstract:

Objective:To observe the effect of the Yiqi Huoxue recipe on the expression of endothelin(ET-1)/interferon-γ(γ-IFN) in radiation-induced lung injury model rats. Method: 66 Wister rats were randomly divided into irradion plus Chinese herbs group (Yiqi Huoxue Recipe group),the purely irrdiated group(model Group),normal contorl group. From the exposure day, the Yiqi Huoxue Recipe group received ig Yiqi Huoxue Recipe 10 g·kg^-1, the model Group and the contorl group received ig distilled water 10 g·kg^-1 everyday,utill the rats were killed. At the 4th , 6th, 8th, 12th, 26th weekend after irradiation, six rats were randomly selected from the Yiqi Huoxue Recipe group and model group. At the same time, six rats in normal group were executed at the 8th weekend. After executing the rats, the serum and right lung tissue were collected for examination. Result: The expression and serum levels of the ET-1 in Yiqi Huoxue Recipe group were lower than those in model Group(P<0.05). γ-IFN began to decrease at the 4th week, and drop at the 12th week and rose again by the 26th week. The expression and serum levels of γ-IFN in Yiqi Huoxue Recipe group were higher than those in model group(P<0.05). Conclusion: Yiqi Huoxue Recipe can regulate and control the expression and secretion of ET-1,γ-IFN, and has good effect on the prevention of radiation-induced lung injury.