清营汤对内毒素致热家兔的作用及其机制

The Effect and Mechanism of the Qingying Decoction on Endotoxin Induced Fever in Rabbits

摘要:

目的: 探讨清营汤对内毒素发热家兔的作用及其机制。 方法: 将48只新西兰大白兔随机分为空白组、模型组、清营汤低、高剂量组(10,20 g·kg^-1),造模前,空白、模型两组分别ig等量生理盐水,清营汤低、高剂量组分别ig不同剂量药物,每日1次,连续5 d,第六日,除空白组外,其余3组分别通过耳缘静脉注入大肠杆菌内毒素方法复制发热家兔模型,观察各组动物症状及体温变化;采用放免法测定血浆血栓素B₂(TXB₂)、6-酮-前列腺素F1α(6-keto-PGF1α)及血清肿瘤坏死因子(TNF-α)的含量;采用发色底物法测定血浆组织纤溶酶原激活物(t-PA)和组织纤溶酶原激活物抑制剂(PAI)的活性。 结果: 与空白组相比,模型组家兔体温,TXB₂,TNF-α,PAI显著上升(P<0.01),6-keto-PGF1α,t-PA显著下降(P<0.01);与模型组相比,清营汤高、低剂量组家兔体温,TXB₂,TNF-α,PAI明显下降(P<0.05或P＜0.01),6-keto-PGF1α,t-PA明显上升(P<0.05或P＜0.01)。清营汤高、低剂量均对内毒素性发热家兔有显著的解热效应(P<0.05或P<0.01),均可明显抑制发热家兔TNFα,TXB₂的含量升高(P<0.05或P＜0.01),降低PAI的活性(P<0.01),提高6-keto-PGF1α的含量和t-PA的活性(P<0.01)。 结论: 清营汤对内毒素性发热家兔有解热化瘀的作用,可能通过抑制发热家兔致热性细胞因子的释放,调节血管内皮细胞的分泌功能而发挥其解热化瘀的作用。

Abstract:

Objective:To investigate the effect and mechanism of Qingying decoction on endotoxin fever in rabbits. Method: First, rabbit fever model was established by Escherichia coli endotoxin. Then rabbits were treated with Qing Ying decoction high dose, low dose to observe the animals symptoms and changes of body temperature; and tumor necrosis factor (TNF-α) content in serum, thromboxane B₂ (TXB₂) and 6-ketone-prostaglandin F1 alpha (6-keto-PGF1α) levels were measured in plasma by radioimmunoassay, and, and plasma tissue plasminogen activator (t-PA), and tissue plasminogen activator inhibitor (PAI) activity were determined by chromogenic substrate method. Result: Qingying decoction of both high dose group and low dose group had a significant antipyretic effect on endotoxin fever in rabbits (P<0.01), could markedly inhibit TNF-α, TXB₂ levels(P<0.05 or P<0.01) and lower PAI activity (P<0.01), increase 6-keto-PGF1α content and t-PA activity (P<0.01)in fever rabbits. Conclusion: Qingying decoction has antipyretic circulation effect on endotoxin fever in rabbits. It may play the role of antipyretic circulation by inhibiting the release of pyrogenic cytokines and regulating the secretory function of vascular endothelial cells in febrile rabbits.