加味生脉散对气虚型鼻咽癌大鼠的放射增敏作用及相关机制

Radiosensitization and Mechanisms of Jiawei Shengmai San on Nasopharyngeal Carcinoma in Rat with Qi Deficient Status

摘要:

目的: 探讨加味生脉散对气虚型鼻咽癌大鼠放疗增敏作用及相关机制。 方法: SD大鼠于玻璃水缸中游泳,每天10 min,共30 d。同时在大鼠腋部皮下注射二亚硝基哌嗪(DNP),剂量为0.5 mg/只/次,每3 d注射1次,共注射30次,于90 d内完成,构建鼻咽癌大鼠模型。实验分为对照组、药物组、放疗组、药物+放疗组,通过测量肿瘤体积及计算抑瘤率,观察药物加味生脉散的放疗增敏作用;荧光定量PCR及Western-blot检测瘤组织多药耐药基因-1(MDR1)基因的表达差异。 结果: 肿瘤质量,对照组(1 230.0±106.8)mg,放疗组为(447.2±121.9)mg,与对照组比较,P<0.05,抑瘤率53.25%;药物加放疗组为(259.9±64.7)mg,与放疗组比较,P<0.05,抑瘤率72.81%;相对表达倍数,药物组为1.21,放疗组为1.85,药物加放疗组为1.34。 结论: 加味生脉散对鼻咽癌的放疗具有增敏作用,其增敏机制可能与抑制MDR1基因的表达相关。

Abstract:

Objective:To investigate radiosensitization and mechanism of Chinese medicine Jiawei Shengmai San on nasopharyngeal carcinoma. Method: The SD rats swimed in Glass tank for 10 minute in 30 days, at the same time,subcutaneous injection of dinitrosopiperazine(DNP) was performed,the dose was 0.5 mg every time,every 3 days the injection was carried out once for 90 days. Then,nasopharyngeal carcinoma models with Qi deficient status was produced.The samples were divided into control group,drug group,radiotherapy group,drug+radiotherapy group.The radiosensitization effect was measured by measuring tumor volume and weight,and multidrug resistance 1 gene(MDR1) expression was detected by quantitative PCR and western-blot. Result: In the control group tumor weight was (1 230.0±106.8) mg,in the radiotherapy group being (447.2±121.9) mg. Compared with the control group,the ratio of tumor inhibition was 53.25%, there was a significant difference (P<0.05). In the drug+radiotherapy group tumor weight was (259.9±64.7) mg,the ratio of tumor inhibition was 72.81%, there also was a significant difference (P<0.05). The Relative expression ratio, in the drug group was 1.21,the radiotherapy group was 1.85,drug+radiotherapy group was 1.34. Conclusion: Jiawei Shengmai San can enhance the sensitivity of radiotherapy to the nasopharyngeal carcinoma,and its mechanisms may be related to regulating MDR1 gene expression.