苦参碱影响豚鼠乳头肌细胞动作电位的实验研究

Influence of Matrine on Cell Action Potential in Guinea Pig Papillary Muscle

摘要:

目的: 观察苦参碱对豚鼠心室肌动作电位的影响,探讨其抗心律失常的作用机制。 方法: 豚鼠分为对照组、苦参碱组(10,50,100 μmol·L^-1)、乌头碱组(1 μmol·L^-1)、乌头碱(1 μmol·L^-1)＋苦参碱(50 μmol·L^-1)组,乌头碱(1 μmol·L^-1)+苦参碱(100 μmol·L^-1)组;采用标准微电极记录技术记录动作电位时程(APD)、动作电位0相幅值(APA)、动作电位50%复极化时程(APD₅₀)和 90%复极化时程(APD₉₀)。 结果: 苦参碱浓度依赖性(10,50,100 μmol·L^-1)适度延长APD₅₀和APD₉₀,降低APA;乌头碱明显延长APD(和对照组比较P<0.05),APD₅₀,APD₉₀ ;苦参碱(100 μmol·L^-1)能纠正乌头碱对APD,APD₅₀,APD₉₀的过度延长作用(和乌头碱组比较P<0.05或P<0.01)。苦参碱(100 μmol·L^-1)能显著降低APA(和乌头碱组比较P<0.05)。 结论: 苦参碱能纠正乌头碱对APD,APD₅₀,APD₉₀的过度延长作用,能使乌头碱诱发心律失常的APA降低,维持动作电位在正常范围可能是苦参碱抗心律失常的机制之一。

Abstract:

Objective:To observe influence of matrine on cell action potential in guinea pig papillary muscles thus to clarify its antiarrhythmic mechanism. Method: Guinea pigs were divided into control group, matrine group(10,50,100 μmol·L^-1),aconitine group 1 μmol·L^-1, aconitine 1 μmol·L^-1＋matrine 50 μmol·L^-1group, aconitine 1 μmol·L^-1+matrine 100 μmol·L^-1 group. Conventional microelectrode technique was used to record action potential duration(APD), action potential amplitude (APA), 50 % and 90 % of APD repolarization(APD₅₀ and APD₉₀). Result: Matrine could prolong moderately APD₅₀ and APD₉₀ in a dose-dependent manner(10,50,100 μmol·L^-1)and reduce APA. Aconitine could excessively prolong APD (vs control group, P<0.05), APD₅₀ and APD₉₀. Matrine(100 μmol·L^-1)could remedy for excessive elonggation of aconitine on APD,APD₅₀ and APD₉₀ (vs aconitine group, P<0.05 or P<0.01). Matrine(100 μmol·L^-1)also could reduce APA(vs aconitine group, P<0.05). Conclusion: Matrine could remedy for excessive elonggation of aconitine on APD,APD₅₀ and APD₉₀ and reduce APA of aconitine-induced arrhythmia, which could maitain action potential in a normal range, and was probably one of matrine antiarrhythmic mechanisms.