抗放射性肺炎方对放射性肺损伤大鼠免疫功能的影响

Effects of Anti-radiation Pneumonia Decoction on Immune Function in Rats with Radiation-induced Pulmonary Injury

摘要:

目的: 探讨抗放射性肺炎方(ARPD)对放射性肺损伤大鼠免疫系统的影响。 方法: 128只SD雄性大鼠随机分成正常对照组(A组)、单纯X射线照射模型组(B组)、地塞米松治疗组(C组)、ARPD治疗组(D组)。B,C,D组麻醉后行直线加速器(6 MV X射线)20 Gy单次胸部照射,C,D组照射后每日分别ig给予地塞米松(1.5 ×10^-3 g·kg^-1·d^-1)和中药(ARPD,35 g·kg^-1·d^-1)治疗。A,B组ig给予等容积生理盐水灌服。照射后第7,15,30,50天取材,观察脾脏组织病理学改变;检测免疫器官指数、外周血淋巴细胞亚群、血清溶血素水平及腹腔巨噬细胞吞噬率。 结果: ARPD治疗组较其他X射线照射组病变程度轻,且恢复快。至第50天,胸腺指数、脾脏指数、CD3⁺,CD4⁺,CD8⁺T淋巴细胞、血清溶血素水平、腹腔巨噬细胞吞噬率ARPD治疗组均高于单纯X射线照射模型组和地塞米松治疗组(P<0.05)。 结论: 抗放射性肺炎方(ARPD)可以提高放射性肺损伤大鼠的免疫功能。

Abstract:

Objective:To explore the effects of anti-radiation pneumonia decoction (ARPD) on immune function in the rats with radiation-induced pulmonary injury. Method: SD rats were randomly divided into four groups: normal control group (group A), model group (group B), dexamethasone treatment group (group C) and ARPD treatment group (group D). Rats in group B, C and D were exposed to 6 MV X-ray at a dose of 20 Gy thoracically. After X-ray exposure group C and D were treated with dexamethasone (1.5 ×10^-3 g·kg^-1·d^-1) and ARPD (35 g·kg^-1·d^-1) respectively by gastrogavage one time a day. The rats in group A and B were gavaged with equal volume 0.9% sodium chloride. The rats were sacrificed at days 7, 15, 30, 50 after X-ray exposure. Pathological changes in spleen tissues were observed and the index numbers of thymus and spleen, T-cell subgroups of peripheral blood, serum hemolysin level, phagocytotic rates of macrophage in abdominal cavity were detected respectively. Result: The pathological changes in group D were slighter and recovered sooner than those in other groups exposed to X-ray. After 50 days, the thymus and spleen indexes, CD3⁺, CD4⁺, CD8⁺, hemolysin levels, phagocytotic rates of macrophage in group D were higher than that in group B and C (P<0.05). Conclusion: ARPD can improve the immune function in the rats with radiation-induced pulmonary injury.