黑骨藤挥发油乳剂抗炎作用及机制

Anti-inflammatory Effects and Mechanism of Emulsion of Volatile Oil from Periploca forrestii

摘要:

目的: 研究黑骨藤挥发油的抗炎作用,阐明挥发油作为药用活性成分的作用机制。 方法: 制备挥发油乳剂,采用大鼠佐剂性关节炎药理模型,设正常对照组、模型对照组、黑骨藤挥发油乳剂高、低剂量(以挥发油量计为375 μL, 94 μL·kg^-1,给药容积均为15 mL·kg^-1)治疗组及阳性药(雷公藤多苷片,6.0 mg·kg^-1) 对照治疗组,造模后,各用药组于致炎后10 d灌胃给药,1次/d,连续给药11 d,正常组及模型组给予等容量(3 mL)蒸馏水,灌胃给药,采用容积法测定其肿胀度;造模后21 d,大鼠眼眶取血,采用丙二醛(MDA)测定试剂盒、一氧化氮(NO)测定试剂盒检测血清中MDA、NO的含量。采用醋酸致小鼠毛细血管通透性实验,设正常对照组、黑骨藤挥发油乳剂高、中、低剂量(500,250,125 μL·kg^-1,给药容积均为20 mL·kg^-1)治疗组及阳性药(地塞米松,4 mg·kg^-1),灌胃给药,每天1次,连续给药5 d,研究黑骨藤挥发油的抗炎作用。 结果: 与模型对照组比较,挥发油高、低剂量组大鼠的足趾肿胀值在18~21 d内均有所降低;与空白组比较,模型组大鼠血清中MDA和NO的含量显著升高(P<0.01),与模型组比较,挥发油高、低剂量组均能降低大鼠血清中的MDA含量,高剂量组大鼠血清中的NO含量明显低于模型组(P<0.05),但与空白对照组并无差异;模型组大鼠血清中的NO含量明显高于空白对照组(P<0.01),对大鼠佐剂性关节炎药理模型有治疗效果,可以明显降低模型大鼠血清中的NO,MDA含量,减轻局部病理反应,起到抗炎作用;黑骨藤挥发油可明显抑制醋酸所致小鼠腹腔毛细血管通透性亢进。 结论: 黑骨藤挥发油具有抗炎作用。

Abstract:

Objective:To study the anti-inflammation effect of volatile oil from Periploca forrestii and the mechanism of action, for providing the basis of the clinical application. Method: The emulsion of volatile oil was prepared. The model of adjuvant arthritis in mice was established with complete freund's adjuvant (CFA) and the changes of nitrogen oxide(NO), malondialdehyde(MDA) content in the serum were determined to estimate the pharmacodynamics of anti-inflammatory activities the in vivo and mouse capillary permeability caused by acetic acid capillary permeability experiment. Rats were randomly divided into control group, model group, positive drug group (tripterygium wilfordii, 6.0 mg·kg^-1), the emulsion of volatile oil high dosage group, low dosage group (the dosage 0.375, 0.094 mL·kg^-1) by gavage administration.Mouses were randomly divided into control group, positive drug group (dexamethasone, 4 mg·kg^-1), the emulsion of volatile oil high dosage group, group,middle dosage group, low dosage group (the dosage 0.500,0.250,0.125 mL·kg^-1). According to a set of drug doses to weight 20 mL·kg^-1 drug gavage. Result: The results indicate that volatile oil from P. forrestii can obviously inhibit the increase of the mouse's abdominal cavity capillary permeability induced by acetic acid, protect against the paws swelling in adjuvant-induced arthritis mice, decrease NO and MDA in the serums of model mice. The volatile oil can alleviate the pathological responses. Conclusion: The volatile oil from P. forrestii had a marked anti-inflammatory effect.