择时合用滋阴、益阳方对肾虚衰老模型HPAT轴的影响

Study of Effects of Time-dependent Combination of Ziyin Prescription and Yiyang Prescription on HPAT Axis of Kidney Deficient Aging Model

摘要:

目的: 比较研究滋阴方六味地黄丸、益阳方金匮肾气丸单用及择时合用对肾虚衰老模型大鼠下丘脑-垂体-肾上腺-胸腺(HPAT)轴的影响。 方法: 大鼠随机分为6组,除正常组外,其余组采用5%D-半乳糖100 mg·kg^-1颈背部皮下注射联合糖皮质激素地塞米松0.5 mg·kg^-1腹腔注射共造模6周,造模两周后,实验第15天开始各给药组分别给予动物ig 1.16 g·kg^-1六味地黄丸、金匮肾气丸、六味地黄丸(早服)合金匮肾气丸(晚服)、金匮肾气丸(早服)合六味地黄丸(晚服),给药4周。比较各组血清皮质醇(COR)、血浆促肾上腺皮质激素(ACTH)、促肾上腺皮质激素释放激素(CRH)含量及外周全血CD4⁺,CD8⁺值。 结果: 与正常对照组相比,肾虚衰老模型组血清COR含量(6.35±1.65)μg·L^-1显著降低(P<0.05),血浆ACTH(53.00±11.86)μg·L^-1,CRH(7.04±1.37)μg·L^-1含量显著升高(均P<0.05),CD8⁺ 水平(23.70±3.93)%显著降低(P<0.01),CD4⁺/CD8⁺(2.73±0.29)显著升高(P<0.01)。与肾虚衰老模型组相比,滋阴益阳组COR含量(10.76±2.09)μg·L^-1显著上调(P<0.01),益阳组、益阳滋阴组ACTH(40.98±8.55),(39.55±14.67)μg·L^-1含量显著下调(P<0.05),滋阴组、滋阴益阳组、益阳滋阴组CRH含量(5.39±1.00),(5.27±0.91),(5.61±1.08)μg·L^-1均显著下调(P<0.05或P<0.01),各用药组CD8⁺水平,CD4⁺/CD8⁺无显著性差异,各组CD4⁺水平无显著性差异。 结论: 滋阴、益阳方单用及择时合用对肾虚衰老模型大鼠HPA轴的影响作用不同,其中以滋阴益阳方的作用较优,但未观察到对免疫功能紊乱的调节作用。

Abstract:

Objective:To observe the effects of the separate application and time-dependent combination of Ziyin prescription(Liuwei Dihuang Pill, LWDHP) and Yiyang prescription (Jingui Shenqi Pill, JGSQP) on hypothalamus-pituitary-adrenal-thymus(HPAT) axis of kidney deficient aging model rats. Method: The rats were randomly divided into six groups. In addition to the normal group, other groups were injected 5%D-galactose solution 100 mg·kg^-1 combined with glucocorticoid dexamethasone solution 0.5 mg·kg^-1 to establish the model for six weeks, after establishing the model two weeks, each medication group was given LWDHP, JGSQP, LWDHP(morning use)combined with JGSQP (night use), JGSQP (morning use) combined with LWDHP(night use),1.16 g·kg^-1 for four weeks, then the content of serum cortisol(COR),plasma adrenocorticotropic hormone(ACTH), corticotropin-releasing hormone(CRH),and CD4⁺,CD8⁺were detected. Result: Compared with natural group, serum COR of kidney deficient aging model group significantly decreased(P<0.05), plasma ACTH, CRH significantly increased(P<0.05),the level of CD8⁺ significantly decreased(P<0.01), CD4⁺/CD8⁺ significantly increased(P<0.01). Compared with kidney deficient aging model group, COR of Ziyin combined with Yiyang group(ZY group) significantly increased(P<0.01), ACTH of Yiyang group, Yiyang combined with Ziyin group(YZ group) significantly decreased(P<0.05), CRH of Ziyin group , ZY group, YZ group significantly decreased(P<0.05 or P<0.01),the level of CD8⁺, CD4⁺/CD8⁺ have no significant differences, the level of CD4⁺ have no significant differences between each group. Conclusion: The effects of separate application and time-dependent combination of Ziyin prescription and Yiyang prescription on HPA axis of kidney deficient aging model rats are different, ZY prescription have the best effect, but these prescriptions have no effect on immunity.