小叶锦鸡儿总黄酮对脑缺血再灌注损伤大鼠的影响

Effect of Total Flavonoid from Caragana microphylla Cerebral Ischemia Reperfusion Injury in Rats

摘要:

目的: 探讨小叶锦鸡儿黄酮类成分对实验性脑缺血再灌注损伤大鼠的作用。 方法: 以正常大鼠作为空白对照组,采用大脑中动脉阻断(MCAO) 制备大鼠局灶性脑缺血再灌注模型,以阿司匹林(12.5 mg·kg^-1)作为阳性对照,小叶锦鸡儿总黄酮(CMF)按照25,50,100 g·kg^-1剂量,灌胃给药4周,观察给药后对MCAO大鼠脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量的影响,并测定其全血黏度和血浆黏度(η),血小板聚集率(PAgT),红细胞聚集指数(EAI),红细胞刚性指数(IR),红细胞变形指数(DI)。 结果: 通过比较各组大鼠血流变学指标η(mPa·s),PAgT(%),EAI,IR,DI时发现,阿司匹林组·s,(56.49±3.74) %,8.83±0.59,3.94±0.22,0.69±0.08)];CMF中剂量组 0.11)mPa·s,(57.67±4.31) %,8.30±0.92,3.99±0.22,0.73±0.06)]、高剂量组(1.57±0.10)mPa·s,(55.59±4.58)%,7.83±0.58,3.65±0.17,0.70±0.07)较模型组大鼠·s,(65.69±4.59)%, 10.75±0.79,4.89±0.17,0.45±0.05)]的η,PAgT显著降低、红细胞参数EAI,IR显著降低,DI升高;通过比较各组大鼠脑组织SOD (U·mL^-1)活性、MDA(nmol·mL^-1)含量时发现,与模型组(50.21±10.74)U·mL^-1,(1.61±1.07)nmol·mL^-1相比,CMF低剂量组(55.45±11.67)U·mL^-1,(1.38±0.96)nmol·mL^-1、中剂量组(60.23±13.59)U·mL^-1,(1.17±0.44)nmol·mL^-1、高剂量组(64.58±9.36)U·mL^-1,(1.08±0.42)nmol·mL^-1均可提高MCAO大鼠脑组织SOD活性,降低MDA含量。 结论: CMF对脑缺血再灌注损伤具有脑保护作用,这可能与其抗氧化应激、降低血黏度有关。

Abstract:

To explore the effect of total flavonoid on cerebral ischemia reperfusion injury in rats. Method: The middle cerebral artery occlusion (MCAO) was used to induce focal cerebral ischemia reperfusion injury model in rats, and the normal Wistar rats were used as control group. After Caragana microphylla Lam total flavonoid (CMF) at dose of 25,50,100 g·kg^-1 and aspirin 12.5 mg·kg^-1 was orally given for 4 weeks, the hemorrheologic parameters including blood viscosity(η), platelet aggregation rate (PAgT), erythrocyte aggregation index(EAI), rigidity index (IR), erythrocyte deformed index (DI), were observed. The contents of superoxide dismutase (SOD) and malonyldialdehyde (MDA) were determined. Result: In terms of the change of hemorheology [η(mPa·s), PAgT(%), EAI, IR, DI], positive control group (1.71±0.08) mPa·s,(56.49±3.74)%,(8.83±0.59),(3.94±0.22),(0.69±0.08), CMF medium group (1.72±0.11) mPa·s,(57.67±4.31)%,(8.30±0.92),(3.99±0.22),(0.73±0.06) and CMF high dose group (1.57±0.10) mPa·s,(55.59±4.58)%,(7.83±0.58),(3.65±0.17),(0.70±0.07) were obviously reduced in η, PAgT, EAI, IR. And they were decreased in DI. Through Comparation of different groups in contents of SOD and MDA, CMF low (55.45±11.67)U·mL^-1,(1.38±0.96)nmol·mL^-1, medium (60.23±13.59)U·mL^-1,(1.17±0.44)nmol·mL^-1, high dose group (64.58±9.36)U·mL^-1,(1.08±0.42)nmol·mL^-1 increased the bioactivity of SOD and decreased the content of MDA. Conclusion: The CMF has a protective effect on cerebral ischemia reperfusion injury in rats; it might be relevant to the role of anti-oxidative stress and decreased blood viscosity.