肉桂多酚改善HepG2细胞胰岛素抵抗的分子机制

Molecular Mechanism of Cinnamon Polyphenols on Improvement of Insulin Resistance in HepG2 Cells in vitro

摘要:

目的: 研究肉桂多酚改善HepG2 细胞胰岛素抵抗的分子机制。 方法: 以HepG2细胞为模型,设立对照组和肉桂多酚实验组,肉桂多酚组设5,10,15 mg·L^-1 3种不同质量浓度组。肉桂多酚作用细胞24 h后,运用实时定量聚合酶链反应(RT-PCR)技术, 检测肉桂多酚对胰岛素抵抗HepG2细胞内葡萄糖转运蛋白2(GLUT2) 、磷酸烯醇丙酮酸羧激酶(PEPCK)和葡萄糖-6-磷酸酶(G-6-Pase)基因表达的影响。 结果: 与对照组比较,肉桂多酚作用细胞24 h后,降低了GLUT2 mRNA的表达(P<0.05);肉桂多酚能够明显降低PEPCK,G-6-Pase mRNA的表达(P<0.05,P<0.01),且随着浓度的升高,肉桂多酚对PEPCK和G-6-Pase mRNA表达的抑制作用越明显。 结论: 肉桂多酚对HepG2 胰岛素抵抗具有明显的改善作用, 其机制可能与降低细胞内GLUT2,PEPCK和G-6-Pase mRNA的表达有关。

Abstract:

Objective:To study the molecular mechanism of cinnamon polyphenols on improvement of insulin resistance in HepG2 cells in vitro. Method: The HepG2 cells were used as cell model. Cells were incubated with cinnamon polyphenols of three different concentrations (5, 10,15 mg·L^-1).Control cells were incubated without cinnamon polyphenols. After 24 h,the mRNA expression of porcine glucose transporter 2 (GLUT2) phosphoenolpyruvate carboxykinase(PEPCK) and glucose-6-phosphatase (G-6-Pase) related to insulin resistance was detected by Real-time PCR(RT-PCR). Result: Compared with the control group, the RT-PCR showed the mRNA expression of GLUT2 decreased after being treated with the cinnamon polyphenols(P<0.05);the cinnamon polyphenols significantly decreased the expression of PEPCK and G-6-Pase mRNA(P<0.05,P<0.01),moreover, the cinnamon polyphenols inhibited the expression of PEPCK and G-6-Pase mRNA in a dose-dependent manner. Conclusion: Cinnamon polyphenols can significantly improve insulin-resistance of HepG2 cells and its mechanism is related to the inhibition of GLUT2,PEPCK, G-6-Pase gene expression.