4个新黄酮类化合物抗中性粒细胞呼吸爆发作用比较

Comparison of Activities of Four Neoflavonoids on Neutrophils Respiratory Burst

摘要:

目的: 比较4个新黄酮类化合物抗中性粒细胞呼吸爆发作用。 方法: 采用化学发光法,以抗坏血酸(V_C)为参照,以IC₅₀作为评价指标,比较4个新黄酮类化合物抗中性粒细胞呼吸爆发作用的强弱关系。 结果: 4个新黄酮类化合物抗中性粒细胞呼吸爆发作用呈对数量效关系,且作用强弱依次为2,4-dihydroxy-5-methoxy-benzophenone,R(+)-dalbergiphenol,R(-)-latifolin,2-O-methyllatifolin。 结论: 新黄酮类化合物分子结构中交叉共轭结构,有利于化合物抗中性粒细胞呼吸爆发能力的提高。A环酚羟基是重要的活性基团,而B环酚羟基和甲氧基的取代可减弱化合物的活性。

Abstract:

Objective:To compare inhibition effect of four neoflavonoids on respiratory burst of rat neutrophils. Method: Vc as reference, chemi-luminescence was used to compare strengths of the four neoflavonoids compounds in antineutrophill rspiratory burst. Result: Four neoflavonoids have different effect on neutrophils respiratory burst, and the marked logarithmic relationship existed between bioactivity and chemical structure. The inhibition abilities of four neoflavonoids on neutrophils respiratory burst were as follows: 2,4-dihydroxy-5-methoxy-benzophenone, R(+)-dalbergiphenol,R(-)-latifolin, 2-O-methyllatifolin. Conclusion: In chemical structure of neoflavonoids,the Cross-Shaped conjugation of A ring was favorable for the inhibition effect of four neoflavonoids on neutrophils respiratory burst, and the phenolic hydroxyl group,in A ring, was the key bioactivity group. In addition, the hydroxymethylation of hydroxyl in B ring was not favorable for improving bioactivity.