补肾化浊方对非酒精性脂肪肝大鼠血清瘦素与胰岛素抵抗的影响

Influence of Bushen Huazhuo Fang on Leptin and Insulin Resistance in Rats with Nonalcoholic Fatty Liver

摘要:

目的: 研究补肾化浊方对高脂饲料喂养大鼠肝功、血脂、血糖、胰岛素抵抗、瘦素的影响,探讨补肾化浊方防治非酒精性脂肪肝作用机制。 方法: 45只Wistar大鼠随机分为空白组、模型组、水林佳(19 g·kg^-1)组和补肾化浊方(含生药20 g·kg^-1)组。空白组予普通饲料喂养,其余3组予高脂饲料喂养,水林佳组、补肾化浊方组在高脂饲料喂养的同时进行相应的药物干预,12周后,处死大鼠,计算大鼠肝指数、Lee’s指数、胰岛素抵抗指数(IRI)、胰岛素敏感指数(ISI),检查肝功能、血脂、血糖、胰岛素(INS)、瘦素(LEP),并进行肝脏病理检查。 结果: 与模型组比较,补肾化浊方组、水林佳组均能明显改善大鼠丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、LEP水平(P<0.01);在改善大鼠INS,IRI,ISI方面,补肾化浊方组优于水林佳组(P<0.05或P<0.01),水林佳组对INS,IRI无明显改善作用,水林佳组、补肾化浊方组大鼠脂肪染色、糖原染色改善优于模型组(P<0.05或P<0.01)。 结论: 补肾化浊方通过保肝、调节血脂、改善瘦素及胰岛素抵抗等方面发挥治疗作用。

Abstract:

Objective:To study the influence of Bushen Huazhuo Fang (BHF) on improving leptin and insulin resistance in rats with nonalcoholic fatty liver, and to explore its mechanism. Method: Forty-five Wistar rats were randomly divided into blank group, model group, silybin capsules(19 g·kg^-1) group and BHF group. The blank group was fed with normal diet, model group, silybin capsules and BHF group were fed with the high fat diet. Silybin capsules and the BHF group were fed with the corresponding drug. After 12 weeks, rats were sacrificed, the rat liver index, Lee’s index, insulin resistance index(IRI), insulin sensitivity index(ISI) were calculated. Liver chemical parameters, blood lipids, blood glucose, insulin(INS), leptin(LEP) and tumor necrosis factor and liver Pathological investigation were carried out and evaluated. Result: BHF and silybin could significantly improve the alanine transaminase(ALT), aspartate transaminase(AST), LEP (P<0.01); BHF was better than silybin for improving INS, IRI, ISI (P<0.05 or P<0.01), Silybin had no significant effect on improving INS and IRI. Silybin and BHF groups were better than the model group in fat staining, glycogen staining (P<0.05 or P<0.01). Conclusion: The therapeutic effect of BHF may come from protecting liver, regulating blood lipids, improving LEP and IRI.