蛇葡萄素诱导人胃癌细胞凋亡的研究

Study of Ampelopsin on Apoptosis in Human Gastric Cancer Cells

摘要:

目的: 研究蛇葡萄素对人胃癌细胞(SGC-7901)凋亡的影响。 方法: MTT比色法测定细胞的增殖抑制率,流式细胞仪Annexin-V FITC/PI双染检测蛇葡萄素33.3,50,75 mg·L^-1作用下SGC-7901细胞的凋亡及各细胞周期。 结果: 蛇葡萄素作用于SGC-7901 48 h后,可明显促进细胞早期凋亡,50,75 mg·L^-1细胞凋亡率为(29.03±6.21)%,(43.43±1.00)%,明显高于空白组(11.83±4.67) %(P<0.05,P<0.01)且呈剂量依赖关系。蛇葡萄素33.3,50,75 mg·L^-1作用于SGC-7901细胞48 h后,G₁期细胞分别为(52.07±4.41)%,(57.07±2.70)%,(58.43±2.66)%明显高于空白对照组(42.00±3.04)%(P<0.05,P<0.01,P<0.01)。 结论: 蛇葡萄素能诱导SGC-7901细胞凋亡并使细胞周期阻滞在G₁期。

Abstract:

Objective:To study ampelopsin on apoptosis in human gastric cancer cells (SGC-7901). Method: Cell proliferation inhibition rate was measured by MTT,The situation in different apoptosis phase was evaluated by flow cytometry (Annexin-V/PI double staining). Result: Ampelopsin could significantly promote early apoptosis in SGC-7901 after 48 h. Ampelopsin of 50 and 75 mg·L^-1 induced apoptosis of (29.03±6.21)%, and (43.43±1.00)% with a dose-dependent relationship, significantly higher than that in the control group (11.83±4.67)% (P<0.05,P<0.01,P<0.01). Cells in G₁ phase was (52.07±4.41)%, (57.07±2.70)%, (58.43±2.66)%, significantly higher than (42.00±3.04)% in control group (P<0.05, P<0.01,P<0.01). Conclusion: Ampelopsin could induce the SGC-7901cell apoptosis and block the cells in G₁ phase.