葛根芩连汤对心律失常模型大鼠血管保护作用的研究

Vascular Protection of Gegen Qinlian Decoction in Rats with Arrhythmias

摘要:

目的: 观察葛根芩连汤(Gegen Qinlian decoction)对心律失常模型大鼠血管保护的作用。 方法: 60只SD大鼠随机分成假手术组、模型组、稳心颗粒组(2.43 g·kg^-1)、葛根芩连汤低(2.16 g·kg^-1),中(4.32 g·kg^-1),高(8.64 g·kg^-1)剂量组,每组各10只,于手术中结扎大鼠左冠状动脉前降支,在心肌缺血30 min再灌注15 min损伤模型上,监测肢体II导联心电图(ECG)。术后放射免疫分析法测定血浆内皮素(ET)、血管紧张素II(AngII)含量;酶联免疫吸附法(ELISA)检测血清肿瘤坏死因子-α(TNF-α)含量和心肌组织核因子-κB(NF-κB)表达量。 结果: 与假手术组相比,模型组大鼠再灌注后AngII,NF-κB,TNF-α和ET水平均升高(P<0.01);与模型组相比,葛根芩连汤组能降低再灌注心律失常发生数(P<0.05),并可减少再灌注后AngII,NF-κB,TNF-α和ET的水平(P<0.01)。 结论: 葛根芩连汤对大鼠再灌注心律失常有一定的保护作用。其作用机制可能通过降低AngII的含量,从而抑制缺血区NF-κB的激活,减少TNF-α和ET有关。

Abstract:

Objective:To observe vascular protection effect of Gegen Qinlian decoction(GQD) in rats with arrhythmia. Method: Sixty rats were randomly divided into six groups: sham group, model group, positive drug group, and three dose groups of GQD (2.16, 4.32, 8.64 g·kg^-1).Ligating the left anterior descending artery for 30 min ischemia and then reperfused for 15 min, limb II ECG was investigated in the isxhemia/reperfusion model. After the operation, postoperative radioimmunoassay was used to detect the content of plasma endothelin (ET) and angiotensin II(Ang II). The tumor necrosis factor-α (TNF-α) of serum and the nuclear factor-κB (NF-κB) in myocardial tissue were measured by using ELISA. Result: Compared with the sham operation group, increased AngII,TNF-α and NF-κB expressions were found in model group rats after reperfusion(P<0.01);Compared with the model group, GQD could significantly reduce reperfusion arrhythmia score (P<0.05),NF-κB,TNF-α and ET could be significantly reduced in GQD groups(P<0.01). Conclusion: GQD has a certain protective effect against reperfusion induced arrhythmia, which is related to reducing the content of AngII and then suppressing the expression of NF-κB,TNF-α,ET in infarcted reflow.