正交试验优选百清凝胶的提取工艺

Optimization of Extraction Technology for Baiqing Gel by Orthogonal Test

摘要:

目的: 优选百清凝胶的提取工艺。 方法: 以丹皮酚含量为指标,单因素试验考察徐长卿的蒸馏提取工艺;以苦参碱、盐酸小檗碱含量和浸膏得率的综合评分为指标,选取浸泡时间、加水量,回流次数和时间为考察因素,采用正交试验优选百清凝胶的提取工艺。 结果: 收集10倍量徐长卿质量的蒸馏液,蒸馏后药渣与苦参、黄柏等药材饮片加10倍量水提取3次,每次1.5 h。 结论: 优选工艺的合理、稳定、且提取率高。

Abstract:

Objective:To optimize extraction technology of Baiqing gel. Method: With the content of paeonol as index,distillation extraction technology of Cynanchum paniculatum was investigated by single factor test;With the content of matrine,berberine hydrochloride and extract yield as comprehensive index,soaking time,the amount of water reflux times and time were chosen as factors,extraction technology of Baiqing gel was optimized by orthogonal test. Result: Distillate was collected with 10 times the amount of C. paniculatum,dregs(after distillation),Phellodendron chinense, Sophora flavescens and other herbal pieces were extracted 3 times with 10 times the amount of water,1.5 h each time. Conclusion: This optimized technology was rational and stable with high extraction rate.