追风伞总黄酮提取工艺优选及其体外抗氧化作用考察

Optimization of Extraction Technology and Investigation of in vitro Antopxidation for Total Flavonoids from Lysimachia paridiformis

摘要:

目的: 优选追风伞中总黄酮的提取工艺,并考察其体外抗氧化作用。 方法: 以总黄酮得率为指标,选取料液比、乙醇体积分数、回流时间与温度为考察因素,采用单因素试验和正交试验优选追风伞总黄酮的提取工艺;采用紫外分光光度法测定追风伞总黄酮对羟自由基和超氧阴离子自由基的清除作用和还原能力。 结果: 追风伞总黄酮的最佳提取工艺为乙醇体积分数65%,料液比1:25,回流温度65℃,回流时间4 h,黄酮得率1.69%;追风伞总黄酮具有较好的还原能力和抑制自由基的能力,且随黄酮质量浓度的增加,还原能力和抑制能力提高。 结论: 追风伞总黄酮具有明显的抗氧化作用。

Abstract:

Objective:To optimize extraction technology of total flavonoids from Lysimachia paridiformis,and investigate its in vitro antopxidation. Method: With yield of total flavonoids as index,single factor and orthogonal test were used to optimized extraction technology of total flavonoids from L. paridiformis with solid-liquid ratio,the concentration of ethanol,reflux time and temperature as factors;Scavenging action and reducing power of total flavonoids on hydroxyl radical and superoxide anion free radical were determined by UV spectrophotometry. Result: Optimum extraction technology of total flavonoids from L. paridiformis was:ethanol concentration 65%,ratio of solid-liquid 1:25,reflux temperature 65℃,extraction time 4 h,yield of total flavonoids of 1.69%;Total flavonoids from L. paridiformis had good reducing power and ability to inhibit free radical,and reducing power and scavenging hydroxyl radical activity increased with increasing the concentration of total flavonoids. Conclusion: total flavonoids from L. paridiformis had significant antopxidation.