正交试验法优选山楂袋泡茶的水提取工艺

Optimization of Water Extraction Technology for Total Flavonoids from Crataegus pinnatifida

摘要:

目的: 优选山楂袋泡茶的浸泡工艺。 方法: 以山楂总黄酮质量浓度为指标,采用紫外分光光度法测定山楂总黄酮含量,通过正交试验考察液料比、浸提时间、浸提温度和粉碎度对山楂袋泡茶浸泡工艺的影响。 结果: 各因素对浸泡工艺的影响顺序为浸提温度>山楂粉碎度>浸提时间>液料比。最佳浸泡工艺为液料比300 mL·g^-1,浸提温度95℃,浸提时间10 min,粉碎度60目,总黄酮得率达9.36 g·L^-1。 结论: 优选的浸泡工艺稳定可行,可用于山楂袋泡茶的服用。

Abstract:

Objective:To optimize water extraction technology of total flavones from Crataegus pinnatifida. Method: Taking yield of total flavonoids as index,the content of total flavonoids was determined by UV,orthogonal test method was used to optimize ultrasonic extraction technology of total flavonoids with extraction time,ratio of solid-liquid,extraction temperature and powder size as factors. Result: Influence sequence of 4 factors on soaking technology was:extraction temperature>powder size>extraction time>ratio of solid-liquid,optimum extraction technology was as following:extraction temperature 95℃,ratio of solid-liquid 1:300,extraction time 10 min, gringing degree 60 mesh,average yield of total flavonoids could be up to 9.36 g·L^-1. Conclusion: Optimized water extraction condition was stable and feasible,it could be used for taking of C. pinnatifida teabag.