流苏金石斛中牡荆素2″-O-β-D-葡萄糖苷的分离鉴定及含量测定

Isolation, Identification and Quantitative Analysis of Vitexin 2″-O-β-D-glucopyranoside in the Whole Herb of Flickingeria fimbriata

摘要:

目的: 分离制备流苏金石斛药材指标性成分, 并建立测定流苏金石斛中该成分含量的高效液相色谱法。 方法: 采用Shiseido(Pak-C₁₈)(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-水(20:80)为流动相,流速1.0 mL·min^-1,检测波长340 nm,柱温25℃。 结果: 从流苏金石斛中分离制备其指标性成分牡荆素2″-O-β-D-葡萄糖苷;该成分在0.016~1.6 μg线性关系良好(r=0.999 8),平均加样回收率为99.66%(RSD 1.05%)。 结论: 方法快速简便,可靠,重复性好,为流苏金石斛药材的质量控制提供依据。

Abstract:

Objective:To prepare chemical marker and establish HPLC method for determining this marker in the whole herb of Flickingeria fimbriata. Method: A Pak-C₁₈(4.6 mm×250 mm,5 μm)column was adopted with a mobile phase of acetonitrile-water (20:80) at the flow rate of 1.0 mL·min^-1 and the column temperature was set at 25℃. The detection wavelength was 340 nm. Result: Vitexin 2″-O-β-D-glucopyranoside was firstly isolated as a chemical marker from F. fimbriata. This marker showed a good linear relationship in the range of 0.016-1.6 μg(r=0.999 8)and the average recovery was 99.66% (RSD 1.05%). Conclusion: The method is quick, simple, reliable, and reproducible, which can be used for quality control of F. fimbriata.