HPLC同时测定裸花紫珠药材中毛蕊花糖苷和木犀草素

Determination of Acteoside and Luteolin in Callicarpa nudiflora by HPLC

摘要:

目的: 建立同时测定裸花紫珠药材中毛蕊花糖苷和木犀草素的方法。 方法: 采用高效液相色谱法,Phenomenex Gemini C₁₈(4.6 mm×250 mm, 5 μm)柱,以甲醇(A)-0.3%磷酸水溶液 (B) 为流动相线性梯度洗脱,流速1.0 mL·min^-1,检测波长350 nm,柱温30℃。 结果: 毛蕊花糖苷和木犀草素线性范围分别在29.6~740.0,1.0~25.0 μg(r=0.999 9),平均回收率分别为99.08%,99.93%,RSD分别为1.53%,1.65%。 结论: 方法精密度高,重复性好,可用于裸花紫珠药材中毛蕊花糖苷和木犀草素的含量测定。

Abstract:

Objective:To establish an HPLC method for determination of acteoside and luteolin in Callicarpa nudiflora. Method: HPLC method was applied on Phenomenex Gemini C₁₈ column (4.6 mm×250 mm, 5 μm) at 30℃ under the condition of gradient elution with mobile A (methanol) and mobile B (0.3% phosphate). The flow rate was 1.0 mL·min^-1, the detection wavelength was set at 350 nm. Result: Two components had a good liner relationship in concentration of 29.6-740.0, 1.0-25.0 μg (r=0.999 9). The average recoveries were 99.08%, 99.93% and the RSD was 1.53%, 1.65% respectively. Conclusion: The method is accurate and reproducible for determination of acteoside and luteolin in C. nudiflora.