HPLC-ELSD同时测定枇杷叶中6种三萜酸成分的含量

Simultaneous Determination of Content of Six Triterpene Acids in Eriobotrya japonica by HPLC-ELSD

摘要:

目的: 建立HPLC-ELSD同时测定枇杷叶中6种三萜酸成分含量的方法。 方法: 采用Ultimate XB C₁₈色谱柱(4.6 mm× 250 mm,5 μm),流动相乙腈-甲醇-0.5%醋酸铵溶液,梯度洗脱,流速1.0 mL·min^-1,柱温35℃,蒸发光散射检测器漂移管温度95℃,氮气流速2.5 L·min^-1。 结果: 蔷薇酸、委陵菜酸、马斯里酸、科罗索酸、齐墩果酸、熊果酸分别在0.436~2.180,0.392~1.960,0.39~1.960,0.856~4.280,0.424~2.120,1.592~7.960 μg,进样质量对数值与峰面积对数值呈良好线性关系;平均回收率分别为98.9%(RSD 3.8%),96.3%(RSD 2.7%),97.7%(RSD 2.6%),97.6%(RSD 3.2%),96.6%(RSD 1.9%),102.5%(RSD 2.5%)。 结论: 建立的含量测定方法简便、准确、分离效果好、重复性好,为更好地控制枇杷叶药材质量提供试验依据。

Abstract:

Objective:To establish an HPLC-ELSD method for simultaneous quantitation of six triterpene acids from Eriobotrya japonica. Method: Samples were analyzed with Ultimate XB C₁₈ column (4.6 mm×250 mm,5 μm) and eluted with acetonitrile-methanol-0.5% ammonium acetate solution as mobile phases in gradient mode,flow rate was 1.0 mL·min^-1,column temperature of 35℃,drift tube temperature of ELSD was set at 95℃,and flow rate of nitrogen was 2.5 L·min^-1. Result: There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0.436-2.180,0.392-1.960,0.39-1.960,0.856-4.280,0.424-2.120,1.592-7.960 μg for rosolic acid,tormentic acid,maslinic acid,corosolic acid,oleanolic acid,ursolic acid,respectively.The average recoveries of these six triterpene acids were 98.9%(RSD 3.8%),96.3%(RSD 2.7%),97.7%(RSD 2.6%),97.6%(RSD 3.2%),96.6%(RSD 1.9%),102.5%(RSD 2.5%),respectively. Conclusion: This established method was simple,accurate and repeatable with good separation,it could provide experimental basis for a better quality control of E. japonica.