芪天胶囊中红景天苷和芍药苷的含量测定

HPLC Determination of Salidroside and Paeoniflorin in Compound Qitian Capsules

摘要:

目的: 建立HPLC测定芪天胶囊中红景天苷和芍药苷含量的方法。 方法: 采用:Kromasil ODS-1色谱柱(4.6 mm×250 mm,5 μm),流动相为甲醇(A)-0.02 mol·L^-1磷酸(B),梯度洗脱[0~15 min,19%A;15~50 min,19~28%A;50~60 min,28%A]分段变波长测定,0~25 min,275 nm(测红景天苷),26~60 min,230 nm(测芍药苷);柱温35℃,流速1.0 mL·min^-1。 结果: 两种成分的线性关系良好,精密度、稳定性、重复性的RSD均<3%,平均加样回收率分别为98.33%,97.77%。 结论: 该方法简单,灵敏、准确。可用于芪天胶囊的质量控制。

Abstract:

Objective:To describe a method for determining of salidroside and paeoniflorin in compound Qitian capsules by HPLC. Method: The determination was carried out over a COSMOSIL column (4.6 mm×250 mm, 5 μm) at the temperature (35℃). The mobile phase consisted of methanol (A)-0.02％ phosphoric (B) solution with the gradient elution (0-15 min, 19%; 15-50 min, 28%A; 50-60 min, 28%A, the flow rate:1 mL·min^-1 and the detection wavelength was set at 275 nm during 0-25 min and 230 nm during 26-60 min. Result: Two regression equation showed good linear relationship between the peak area ratio of each marker to internal standard and amounts. The average recovery of salidroside was 98.33% (RSD 0.71%), of paeoniflorin was 97.77% (RSD 1.18%). Conclusion: The method is simple, accurate, sensitive, and can be used for determination of two components in Qitian capsules with satisfactory accuracy and repeatability.