白洋淀芦根药材中阿魏酸UPLC含量测定

Determination of Ferulic Acid in Pharagmitis Rhizoma Produced in Baiyangdian by UPLC

摘要:

目的: 建立白洋淀芦根药材的UPLC含量测定方法。 方法: 对10批白洋淀产的芦根药材采用70%甲醇为溶剂超声提取制备溶液,应用WATERS UPLC超高效液相色谱仪,BEH C₁₈色谱柱,PDA检测器进行测定。以乙腈-水为流动相进行梯度洗脱,流速0.1 mL·min^-1,柱温25℃,检测波长320 nm。 结果: 阿魏酸在0.038~0.38 μg呈良好的线性关系(R²=0.999 9)。白洋淀产芦根中阿魏酸的含量在0.051%~0.169%。 结论: 建立的白洋淀芦根药材中阿魏酸的UPLC含量测定方法快速、高效,为白洋淀芦根药材的质量控制提供了有效手段。

Abstract:

Objective:To establish UPLC determination method of Phragmitis Rhizoma in Baiyangdian. Method: Ten samples of Phragmitis Rhizoma in Baiyangdian from different place were extracted by 70% methanol with ultrasonic extractor. Sample solutions were determined by Waters UPLC equipped with BEH C₁₈ column and PDA detector, gradient eluted with acetonitrile-water as mobile phase. The flow rate was set to 0.1 mL·min^-1, while the column temperature was set at 25℃, and the wavelength for detection was set to 320 nm. Result: The standard curve was in good linearity over the range of 0.038-0.38 μg (R²=0.999 9). Conclusion: The method established is simple, accurate and can be used to control the quality of Pharagmitis Rhizoma producted in Baiyangdian.