HPLC测定地榆配方颗粒中没食子酸的含量

Determination of Content of Gallic acid in Sanguisorba Granule by HPLC

摘要:

目的: 建立高效液相色谱法测定地榆配方颗粒中没食子酸的定量分析方法,为制定其质量标准及开发利用提供科学依据。 方法: 色谱柱:Diamonsil C₁₈(2)柱(4.6 mm×250 mm,5 μm),流动相甲醇-0.05%磷酸(5:95),检测波长272 nm,柱温20℃。 结果: 在上述色谱条件下,没食子酸进样量在0.009 72~0.097 2 mg·kg^-1与其峰面积成良好的线性关系,回归方程Y=34 347.2X+10.887(r=0.999 9);平均加样回收率为99.83%,RSD 2.3%(n=6)。 结论: 该测定方法简便易行,结果准确,重复性好,可用于地榆配方颗粒中没食子酸的含量测定,并为其提供了有一定参考价值的实验数据。

Abstract:

Objective:To establish the quantitative analysis method for determining the content of gallic acid in Sanguisorba Granule by HPLC so as to provide a scientific basis for development of the drug as well as establishment of its quality standards. Method: Diamonsil C₁₈(2) column (4.6 mm×250 mm,5 μm) was used with methanol-0.05% phosphoric acid (5:95) as the mobile phase; the detection wavelength was 272 nm, the velocity of flow was 1.0mL·min^-1, and the column temperature was 20℃. Result: Under the above chromatographic conditions, the injection volume of gallic acid showed a linear relationship with peak area when ranging between 0.009 72-0.097 2 mg·kg^-1, the corresponding regression equation being Y=34 347.2X+10.887 (r=0.999 9); the average recovery of gallic acid was 99.83% with corresponding relative standard deviation (RSD) being 2.3% (n=6). Conclusion: The method is simple, accurate, reproducible and can be used for determination of the content of gallic acid in Sanguisorba Granule.