HPLC测定刺梨中杨梅素和槲皮素的含量

Determination of Myricetin and Quercetin in Rose roxburghii by HPLC

摘要:

目的: 建立同时测定中药刺梨中杨梅素和槲皮素含量的HPLC分析方法。 方法: 采用Zorbax SB-C₁₈色谱柱(4.6 mm×150 mm,5 μｍ),以甲醇-0.4%磷酸溶液为流动相,流速1.0 mL·min^-1;检测波长368 nm。 结果: 杨梅素和槲皮素分别在0.036 2~0.253 7μg,0.056~0.196 μg峰面积与浓度呈良好的线性关系(r=0.999 9);平均加样回收率分别为98.32%,98.84%,RSD分别为2.18%,1.78%。 结论: 该方法简便、准确、重现性好,可用于中药刺梨的质量控制。

Abstract:

Objective:To develop a high performance liquid chromatographic method for simultaneous determination of myricetin and quercetin in Rose roxburghii. Method: The optimized method was achieved for the separation and detection of myricetin and quercetin using Zorbax SB-C₁₈ (4.6 mm×150 mm, 5 μm) as the stationary phase, methanol-0.4% aqueous phosphoric acid solution (65:35) as the mobile phase at a flow rate of 1.0 mL·min^-1, 368 nm as the detection wavelength. Result: Myricetin and quercetin showed good relationship in the range of 0.036 2-0.253 7 μg and 0.056-0.196 μg, respectively. The average recoveries of myricetin and quercetin were 98.32% and 98.84% with relative standard derivation (RSD) of 2.18% and 1.78%. Conclusion: The results showed that the method is simple, accurate and repeatable and it is suitable for quality control of R. roxburghii.