辛夷药材(望春花)不同栽培品种质量分析

Research on Quantitative Determination of Volatile Oil in Different Cultivated Species of Magnoliae Flos (Magnolia biondii)

摘要:

目的: 建立辛夷药材(望春花)挥发油的含量测定及以桉油精为指标的气相色谱测定的质量分析方法。 方法: 应用《中国药典》的挥发油测定方法对望春花10个主流栽培品种的挥发油进行含量测定;应用气相色谱法对望春花10个主流栽培品种的桉油精进行含量测定。采用HP-5二苯基聚硅氧烷共聚物(0.25 μm× 0.32 mm×30 m)毛细管柱,FID检测器,载气为高纯度氮气,程序升温:初始温度80℃保留6 min,以20℃ min^-1的速率升温至160℃,保留2 min;再以60℃ min^-1的速率升温至220℃,保留5 min;进样口温度240℃,检测器温度240℃。 结果: 挥发油含量依次为3.24%,4.64%,6.12%,3.64%,5.48%,6.98%,3.64%,3.24%,7.68%,2.88%。气相色谱测定桉油精含量:桉油精的进样量在1.1 ~10.45 μg(r=0.999 8)线性关系良好,平均回收率为100.02%,RSD 1.12%;含量依次为2.076%,4.212%,3.689%,0.104 1%,1.590%,3.523%,2.930%,1.940%,4.520%,1.494%。 结论: 根据望春花的挥发油及以气相色谱法桉油精的含量测定,显示不同栽培品种的望春花挥发油和桉油精的含量都有一定的差别,可用于望春花不同栽培品种质量评价和质量标准的制定。

Abstract:

Objective:To establish a method for the determination of volatile oil and the determination of eucalyptol in volatile oil as an indicator of Magnoliae Flos (Magnolia biondii) by GC. Method: The content of volatile oil of the mainstream 10 cultivars of M. biondii was determined by determination of volatile oil from the 2010 edition of Chinese Pharmacopoeia, and the content of eucalyptol of the mainstream 10 cultivars was determined by GC. The GC-MS conditions with HP-5MS column (0.25 μm×0.32 mm×30 m) was used, FID detector, the hold air consisted of high purity He, temperature programmed: initial temperature 80℃, retain 6 min, rise temperature to 160℃ at the rate of 20℃·min^-1, retain 2 min; rise temperature to 220℃ at the rate of 60℃·min^-1, retain 5 min; inlet temperature 240℃; detector temperature 240℃. Result: The content of volatile oil: 3.24%,4.64%,6.12%,3.64%,5.48%,6.98%,3.64%,3.24%,7.68%,2.88%. Content of eucalyptol determined by GC: the linear range was 1.1-10.45 μg(r=0.999 8); the average recovery was 100.02%,RSD 1.12%. The content of volatile oil: 2.076%,4.212%,3.689%,0.1041%,1.590%,3.523%,2.930%,1.940%,4.520%,1.494%. Conclusion: According to the volatile oil content and the content of eucalyptol determined by GC, there were differences in different cultivars of M. biondii, which provided scientific basis for the quality assessment and quality control standards of different cultivated species of M. biondii.