HPLC-ELSD同时测定链荚豆中胡萝卜苷和β-谷甾醇含量

Simultaneous Determination of Daucosterol and β-sitosterol in Alysicarpus vaginalis by HPLC-ELSD

摘要:

目的: 建立同时测定链荚豆中胡萝卜苷和β-谷甾醇含量的方法。 方法: 采用高效液相色谱-蒸发光散射检测法,色谱柱为Diamonsil C₁₈ 柱(4.6 mm×150 mm,5 μm),流动相甲醇-水(95:5),流速1.0 mL·min^-1,漂移管温度80℃,载气体积流量2.5 L·min^-1。 结果: 胡萝卜苷和β-谷甾醇浓度分别在10~100 mg·L^-1(r=0.999 3)和25~250 mg·L^-1(r=0.999 1)线性关系良好;平均加样回收率分别为99.5%,98.9%,RSD分别为2.4%,2.1%。 结论: 该法专属、简便、准确,可用于同时测定链荚豆中胡萝卜苷和β-谷甾醇,为链荚豆的质量控制提供依据。

Abstract:

Objective:To develop a new method for simultaneous determination of daucosterol and β-sitosterol in Alysicarpus vaginalis. Method: HPLC-ELSD method was used to determine daucosterol and β-sitosterol. The separation was performed on a Diamonsil C₁₈ column (4.6 mm×150 mm, 5 μm) with methanol-water (95:5) as the mobile phase at the flow rate of 1.0 mL·min^-1. The temperature in drift tube was 80℃ and the carrier gas flow was 2.5 L·min^-1. Result: The linear ranges of daucosterol and β-sitosterol were 10-100 μg·mL^-1 (r=0.999 3) and 25-250 mg·L^-1 (r=0.999 1), respectively. The average recoveries were 99.5%, 98.9%, and RSDs were 2.4% and 2.1%, respectively. Conclusion: This method is selective, simple, accurate, which can be applied for simultaneous determination of daucosterol and β-sitosterol in A. vaginalis.