HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量

Content Determination of Ginsenosides and Vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii by HPLC

摘要:

目的: 建立复方西洋参泡腾片中人参皂苷Rg₁、Re、Rb₁及维生素C的含量测定方法。 方法: 采用高效液相色谱法,色谱柱为Diamonsil C₁₈柱(4.6 mm×250 mm,5 μm),人参皂苷及维生素C的流动相分别为乙腈-0.1%磷酸溶液(梯度洗脱)和甲醇-0.1%磷酸溶液(3:97),检测波长203 nm和254 nm。 结果: 人参皂苷Rg₁线性范围0.050 4~1.26 μg,平均加样回收率98.59%;人参皂苷Re线性范围0.336~8.4 μg,平均加样回收率99.11%;人参皂苷Rb₁线性范围1.052~26.3 μg,平均加样回收率98.12%;维生素C线性范围0.806 4~20.16 μg,平均加样回收率97.50%。 结论: 建立的方法简便、重复性好、准确度高,可用于该产品的质量控制。

Abstract:

Objective:To establish the content determination method for ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii. Method: HPLC method was adopted. The chromatographic column was Diamonsil C₁₈ (4.6 mm×250 mm, 5 μm) column. The mobile phases were acetonitrile-0.1% phosphate solution (gradient elution) and methanol-0.1% phosphate solution (3:97) respectively. The detection wavelengths were set at 203 nm and 254 nm. Result: The concentration have good linear correlations within the range of 0.050 4-1.26 μg for notoginsenoside Rg₁, 0.336-8.4 μg for ginsenoside Re, 1.052-26.3 μg for notoginsenoside Rb₁ and 0.806 4-20.16 μg for vitamin C. The average recovery rates were 98.59%, 99.11%, 98.12% and 97.50% respectively. Conclusion: The method is simple, reproducible and accurate, and applicable for the quality control of the product.