连钱草中黄酮、有机酸类成分的HPLC指纹图谱

Studies on Fingerprint of Glechoma longituba Flavone and Organic Acid Compounds by HPLC

摘要:

目的: 建立连钱草药材中黄酮、有机酸类成分的HPLC 指纹图谱分析方法,为整体控制和评价连钱草药材的质量提供依据。 方法: 采用大连依利特 Hypersil BDS C₁₈ (4.6 mm×250 mm,5 μm),流动相为甲醇-0.1%磷酸梯度洗脱,流速1.0 mL·min^-1,检测波长330 nm。 结果: 建立了连钱草药材黄酮、有机酸类成分的HPLC指纹图谱,标定了17个共有峰,其精密度、稳定性、重复性均符合指纹图谱技术要求(RSD<5%),13批连钱草药材的相似度在0.713~0.987。 结论: 该指纹图谱方法简便、准确、重复性好,可作为连钱草药材质量控制的依据。

Abstract:

Objective:To establish a method of flavone and organic acid compounds by HPLC fingerprint, providing the basis for whole control and evaluating the quality of medicinal materials. Method: The separation was performed on an BDS C₁₈(4.6 mm×250 mm, 5 μm) column of Hypersil. The mobile phase was methanol-0.1% phosphoric acid solution, gradient elution. The flow rate was 1.0 mL·min^-1 and the wavelength was set at 330 nm. Result: HPLC fingerprint of flavone and organic acid compounds was established. found 17 co-possessing peaks, RSD of precision and reproducibility was in the range of<5%, and the similarity of 13 batches of Glechoma longituba (Nakai) Kupr was between 0.713-0.987. Conclusion: This method is simple and accurate, reproducible and can be used to provide a base for the quality of the herbs.