野菊花多糖的分离纯化及化学组成研究

Purification and Chemical Composition of Polysaccharides from Chrysanthemum indicum

摘要:

目的: 从野菊花中分离纯化多糖,并对其进行相对分子质量测定及单糖组成分析。 方法: 经热水提取、乙醇沉淀、大孔吸附树脂LSA-21脱色与Sevag法除蛋白,再经DEAE-52纤维素柱和 Sephadex G75 凝胶色谱分离纯化得到野菊花多糖。采用高效凝胶过滤色谱法(HPGPC)分析其相对分子质量,并借助衍生化气相色谱法(GC)对其单糖组成进行解析。 结果: 从野菊花中得到3个均一多糖CIP-1',CIP-2'和 CIP-3'。 结论: CIP-1'的相对分子质量为8 242 Da,由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖6种单糖组成,摩尔比为0.75:1.48:1.96:0.72:1.80:3.00;CIP-2'的相对分子质量为8 383 Da,由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖6种单糖组成,摩尔比为1.17:1.19:0.23:0.56:1.70:2.20;CIP-3'的相对分子质量为19 201 Da,由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖5种单糖组成,摩尔比为2.40:1.43:3.84:7.45:5.10。红外光谱表明,三者均为吡喃糖。

Abstract:

Objective:To study on the purification and chemical composition of polysaccharides from Chrysanthemum indicum. Method:Polysaccharides were extracted from C. indicum with hot water, followed by precipitating with ethanol, decolorizing with LSA-21 and removing of free protein by the method of Sevag, and finally separated into three fractions CIP-1'、CIP-2'and CIP-3'by DEAE-52 cellulose anion exchange and Sephadex G75 gel column chromatograph. Result:The relative molecular weight (Mw) of CIP-1'was estimated to be about 8,242 Da by using high-performance gel permeation chromatography (HPGPC),while that of CIP-2'was 8,383 Da and of CIP-3'was 1.920 1×10³ Da. According to GC analysis, CIP-1'was composed of six kinds of monosaccharides, namely rhamnose, arabinose, xylose, mannose, glucose and galactose in the molar ratio of 5.44:16.40:20.35:3.53:15.43:21.46, following that CIP-2'was composed of the same kinds of monosaccharides in the molar ration of 8.47:13.17:2.43:2.75:14.51:15.76. However, CIP-3'was composed of rhamnose, arabinose, mannose, glucose and galactose in a molar ratio of 17.46:15.88:18.90:63.76:36.44. The FT- IR spectrum of the three also indicated typical characteristics of polysaccharides. Conclusion:This study provided experimental basis for the development of polysaccharide resources from C. indicum.