SD-HS-SPME-GC-MS分析华中碎米荠挥发性成分

Analysis of Volatile Constituents in Cardamine urbaniana by SD-HS-SPME-GC-MS

摘要:

目的: 对华中碎米荠挥发性成分进行研究。 方法: 分别采用水蒸气蒸馏法(SD)和顶空固相微萃取法(HS-SPME)提取华中碎米荠挥发性成分;通过气质联用(GC-MS)分析其化学成分组成,并应用色谱峰面积归一化法计算各成分的相对百分含量。 结果: 采用SD-GC-MS共分离出67个色谱峰,鉴别出其中34个化合物,占挥发油总量的94.34%;采用HS-SPME-GC-MS共分离出62个色谱峰,鉴别出其中48个化合物,占总挥发性成分的98.29%。通过这两种技术共同鉴定出的挥发性成分共有5种,其中含量最高的成分为4-异硫代氰酰基-1-丁烯。 结论: SD与HS-SPME分别适用于不同类型的挥发性成分的提取,两者联用分析华中碎米荠挥发性成分能够得到更加全面的信息。

Abstract:

Objective:To analyze the volatile constituents in Cardamine urbaniana. Method: The volatile oil from C. urbaniana was extracted by steam distillation (SD) and solid phase micro extraction (SPME). The constituents were separated and analyzed by gas chromatography-mass spectrometry with the area normalization method to determine the relative percentage content of each component. Result: Sixty-seven compounds were isolated and 34 compounds were identified by SD that composed about 94.34% of the total volatile oil; and 62 compounds were isolated and 48 compounds were identified by SPME that composed about 98.29% of the volatile oil. Five kinds of the volatile components are identified by the two methods together and the content of 4-isothiocyanato-1-butene is highest. Conclusion: SPME and SD were applied to the extraction of different types of volatile components, combined with the two methods together to analyze the volatile components in C. urbaniana can get more comprehensive information.