干姜中挥发性成分的GC指纹图谱研究

GC Fingerprint of Volatile Constituents of Rhizoma Zingiberis

摘要:

目的: 建立干姜中挥发性成分的指纹图谱,评价不同产地干姜药材的质量差异。 方法: 用水蒸气蒸馏法提取,GC法分析对比11批干姜药材挥发油的成分。色谱条件: HP-5(320 μm×30 m,0.25 μm),程序升温:起始温度60℃,以 5℃·min^-1 程序升温至145℃,保持6 min,再以2℃·min^-1程序升温至165℃,保持5 min,再以8℃·min^-1程序升温至250℃,载气为氮气,流速1 mL·min^-1,分流比30:1;进样口温度250℃,检测器温度250℃。 结果: 标示出干姜挥发油图谱中20个共有峰,11批干姜药材的挥发油相似度较高。 结论: 该指纹图谱方法简便,重复性好,可作为干姜药材的质量控制方法之一。

Abstract:

Objective:To establish chromatographic fingerprint of volatile oil and investigate the variance of Rhizoma Zingiberis collected from different places. Method: First, gather Rhizoma Zingiberis from different places, then smashed and dried out. Second, mark off the different samples and extract the volatile constituents. Third, assay the final samples by GC and correct their fingerprints and make evaluations at last. The chromatography analysis was carried out on an HP-5 capillary column (320 μm×30 m,0.25 μm), the temperature program was as follows: 60℃ for six minutes, rose to 146℃ at 5℃·min^-1, then rose to 165℃ at 2℃·min^-1 for five minutes, and then rose to 250℃ at 8℃·min^-1, by using helium as the carries gas, the flow rate of 1 mL·min^-1, the split ratio 30: 1, sample inlet temperature of 250℃. Result: A total of twenty common peaks identified from the GC fingerprints of volatile oil in Rhizoma Zingiberis.More than eleven samples of representative Rhizoma Zingiberis were chosen for preliminary fingerprint assay. The similarity, repetitiveness, steadiness and precision of these different Rhizoma Zingiberis were obtained. Conclusion: The quality of most of these Rhizoma Zingiberis is stable and controllable, which meet the requirements of the 2005-edition Pharmacopoeia. However, a minority of these Rhizoma Zingiberis has some differences in quality, and requires further investigation.