朱砂、朱砂安神丸及氯化汞在小鼠体内吸收、分布对比

Comparison and Research of Cinnabar, Zhusha Anshen Wan and HgS on Rat Absorption and Distribution

摘要:

目的: 对比研究朱砂、朱砂安神丸及氯化汞在小鼠体内的吸收、分布规律。 方法: 昆明种小鼠180只,分为正常组、朱砂临床剂量组(0.2 g·kg^-1, ig)、朱砂高剂量组(11.2 g·kg^-1, ig)、朱砂安神丸组(2 g·kg^-1, ig)、氯化汞组(0.07 g·kg^-1, ig),每组小鼠在给药1,2,4,8,16,24 h后各取6只鼠的血、脑、肝、肾,原子荧光光度法检测汞含量。 结果: 朱砂临床剂量组和朱砂安神丸组体内最大血药浓度及各时间点血药浓度与氯化汞组相比具有明显差异(P<0.05);朱砂临床剂量组与朱砂安神丸组各时间点肝、肾组织中汞含量与氯化汞组有显著性差异(P<0.05);朱砂临床剂量组和朱砂高剂量组在血液和组织中汞含量亦有显著性差异(P<0.05);朱砂高剂量组脑中汞含量最高。 结论: 临床剂量的朱砂、朱砂安神丸的汞吸收及各组织的汞含量均明显低于高剂量的朱砂和氯化汞。

Abstract:

Objective:To study the regulation of Cinnabar, Zhusha Anshen wan(ZSASW) and HgS on absorption and distribution in mice. Method: Oral gavage was given to Kunming mice with cinnabar (0.2 g·kg^-1), high dose of cinnabar (11.2 g·kg^-1), ZSASW (2 g·kg^-1), HgS (0.07 g·kg^-1) or saline daily for 1, 2, 4, 8, 16, 24 hours, mercury accumulation in blood, liver, kidney and brain was determined by atomic fluoresence detector. Result: In comparison, the maximal serum concentration and serum concentration in each time of cinnabar clinical dose group and ZSASW group have obvious difference with the HgCl₂ group. The mercury accumulation in liver and kidney in each time of clinical dose group and ZSASW group have obvious difference with the HgCl₂ group. The mercury accumulation in blood and organs also had obvious difference between cinnabar clinical dose group and high dose group, and the high dose has the maximal accumulation in brain. Conclusion: Both cinnabar clinical dose group and ZSASW group have obvious difference with cinnabar high dose group and HgS group in absorption and mercury accumulation in organs.