延胡索总生物碱大鼠灌胃给药后入血成分的定性研究

Constituents Absorbed in Rat Serum after Oral Administration of Rhizoma Corydalis Extraction with HPLC-MS-MS

摘要:

目的: 对延胡索提取物给药后大鼠血浆进行成分的定性研究。 方法: 应用ABI 4000 Q TRAP质谱,结合参考文献和对照品对延胡索提取物的入血成分进行定性研究。 结果: 从大鼠血浆中共分析鉴定了8个成分,其中根据已知对照品确定的2个化合物,推测未知的化合物6个。 结论: 大鼠口服延胡索提取物后血浆含有延胡索乙素、脱氢紫堇碱、四氢黄连碱,紫堇杷明碱、异紫堇杷明碱、小檗碱、延胡宁和巴马汀/去氢紫堇鳞茎碱。

Abstract:

Objective:To obtain chemical constituent information of rat serum after oral administration of Rhizoma Corydalis extraction. Method: ABI 4000 Q TRAP Mass was applied to determine the serum of rat and the compounds were analyzed with mass combined with references and standards. Result: Eight compounds in rat serum were identified. Conclusion: They are tetrahydropalmatine, dehydrocorydaline, tetrahydrocoptisine, corypalmine, isocorypalmine, berberine, yuanhunine and palmatine/dehydrocorybulbine.