三因素析因设计分析黄芪有效部位的交互关系

Analysis of Interaction of the Effective Fraction of Astragalus membranaceus Based on Three Factor Factorial Design

摘要:

目的: 采用2×2×2析因设计分析黄芪有效部位(黄芪黄酮、黄芪多糖、黄芪皂苷)干预失血性贫血模型小鼠的效应机制及其交互关系。 方法: 将108只小鼠随机分为9组,每组12只。除正常组外,各组小鼠分别于第1,4,7天进行眶静脉放血,放血量为0.5 mL·20 g^-1,正常组、模型组ig给予蒸馏水10 mL·kg^-1,其余各给药组分别按照0.166,0.445,0.611,0.069,0.235,0.514,0.680 g·kg^-1剂量ig黄芪有效部位药物。运用析因设计方差分析方法对外周血红蛋白(Hb)浓度、红细胞(RBC)计数、红细胞压积(Hct)、造血因子等指标进行方差分析。 结果: 模型组与正常组比较,外周血Hb,RBC,Hct,血清、肾组织促红细胞生成素(EPO),血清特异转录因子(GATA-1),骨髓干细胞因子(SCF)等指标差异均有统计学意义(P<0.05)。黄酮、多糖、皂苷对外周血象作用显著(P<0.05),黄酮与皂苷合用、多糖与皂苷合用效果均优于单一用药;黄酮、多糖对血清EPO浓度,多糖、皂苷对肾组织EPO浓度有影响,以多糖与皂苷的配伍组合效果最佳;黄酮、多糖对血清GATA-1浓度、骨髓SCF浓度有影响,以黄酮作用效果最好。 结论: 多糖与皂苷配伍是改善失血性贫血小鼠外周血象,提升血清及肾组织EPO浓度的最佳配伍组合;黄酮增加骨髓SCF、血清GATA-1浓度作用最佳。

Abstract:

Objective: The factorial design method of effective parts of Astragalus 2×2×2 (Astragalus flavonoids, polysaccharides of astragalus and Astragalus saponin) was used analyze intervention of effect and mechanism in hemorrhagic anemia model mice and their interaction. Method: One hundred and eight mouse randomly were divided into 9 groups, 12 each group. In addition to normal group, orbital venous bleeding was executed at 1, 4,7 day, rasprctively, bloodletting is 0.5 mL·20 g^-1, normal groups, model groups and intragastric infusion were given with distilled water 10 g·kg^-1, other groups were given corresponding drugs with dose of 0.166, 0.445, 0.611, 0.069, 0.235, 0.514, 0.680 g·kg^-1. Factorial design analysis of variance methods was used to analyze peripheral hemoglobin (Hb) concentration, red blood cell (RBC) count, hematocrit (Hct), hematopoietic factors such as indicators for analysis of variance. Result: Compared with normal group, peripheral blood Hb, RBC, Hct, erythropoietin(EPO), serum transcription factor(GATA-1) in serum and kidney tissue, bone marrow, such as stem cell factor(SCF) index differences are statistically significant in model group(P<0.05). Flavone,polysaccharide and saponins glycoside had an obvious effect on peripheral blood (P<0.05), combined application of flavone and saponins glycoside and polysaccharide and saponins glycoside showed a better action than single medication; flavone and polysaccharide had influence on serum EPO, GATA-1 and bone marrow SCF concentration, polysaccharide and saponins glycoside had effect on kidney organization EPO concentration. Conclusion: Combination of polysaccharides and saponins can improve peripheral blood in anemia mice picture.