糖络宁对糖尿病大鼠坐骨神经MnSOD,CuZnSOD,GPx抗氧化酶基因表达的影响

Effects of Tangluoning Formula on Gene Expression of MnSOD, CuZnSOD and GPx in Sciatic Nerve of Diabetic Rats

摘要:

目的: 初步探讨中药复方糖络宁改善氧化应激的分子机制。 方法: 8周龄雄性SD大鼠随机选取15只为正常对照组,余大鼠禁食12 h后腹腔注射链脲佐菌素(STZ)60 mg·kg^-1建立糖尿病模型,72 h后大鼠禁食8 h断尾取血,测定血糖≥16.7 mmol·L^-1视为糖尿病(DM)模型建立成功。成功后随机分为模型组(M组)、糖络宁10 g·kg^-1·d^-1组(TLN组)、α-硫辛酸0.02 g·kg^-1·d^-1组(LA组)各15只,连续给药8周后,采用实时定量荧光PCR(Real-time PCR)技术检测各组大鼠坐骨神经锰超氧化物歧化酶(MnSOD)、铜锌超氧化物歧化酶(CuZnSOD)、谷胱甘肽过氧化物酶(GPx)基因表达量,动态监测血糖、冷热刺激反应时间和热痛阈值。 结果: STZ建立糖尿病模型后,坐骨神经组织的抗氧化酶MnSOD,CuZnSOD,GPx基因表达均增加;糖络宁连续给药8周后,冷热刺激反应时间明显缩短(P<0.05),痛阈值明显延长(P<0.05),MnSOD, CuZnOD, GPx基因表达量均明显升高(P<0.05)。 结论: 糖络宁能够改善STZ糖尿病大鼠痛觉过敏、感觉迟钝症状,并能通过提高坐骨神经MnSOD, CuZnSOD,GPx基因表达减轻氧化应激。

Abstract:

Objective:To explore possible molecular mechanism of Tangluoning(TLN) formula in improving oxidative stress. Method: The SD rats were divided into control group, the others were given intraperitoneal injection of streptozocin(STZ) (60 mg·kg^-1) after 12 hours of fasting to build diabetic model. The rats with blood glucose level being more than 16.7 mmol·L^-1 after stop 8 hours of fasting were regarded as modelling successfully after 3 days.The rats modeling successfully were randomly divided into the model group, TLN group and alpha-lipoic acid group (LA), 15 in each group. Drugs were administrated orally for 8 weeks after the STZ treatment.Blood glucose, cool or thermal response time,thermal threshold were determined during the experimental process. After eight weeks of treatment,Real-time PCR technology was employed to measure Mn superoxide dismutase(SOD) mRNA,CuZnSOD mRNA and glutathione peroxidase(GPx) mRNA expression in the sciatic nerve. Result: The expression of MnSOD mRNA,CuZnSOD mRNA and GPx mRNA was increased after STZ treatment. Compared with the model group,the cool or thermal response time was decreased, the thermal threshold was increased, the gene expression of MnSOD,CuZnSOD and GPx was increased by TLN. Conclusion: Tangluoning formula could improve hyperalgesia, dysesthesia,increase antioxidant enzymes gene expression to improve oxidative stress.