小半夏加茯苓汤水提物特征图谱及其止吐药效物质基础的研究

Study on Characteristic Chart of Xiaobanxia Plus Fuling Decoction Extract and Basic Substances of its Antiemetic Function

摘要:

目的: 建立小半夏加茯苓汤的特征图谱,探索小半夏加茯苓汤止吐作用的有效物质基础。 方法: 采用HPLC建立小半夏加茯苓汤的特征图谱。实验分3批,每批取健康家鸽50只,均分为空白对照组、呕吐模型组、小半夏加茯苓汤(拆方、生半夏)高、中、低剂量组(0.6,0.3,0.15 g·kg^-1),按分组给药,对照组和模型组给予同体积蒸馏水,每天1次,连续3 d。末次药后1 h用硫酸铜制作家鸽呕吐模型,以呕吐潜伏期、呕吐次数、呕吐频率为指标,观察小半夏加茯苓汤(原方)、拆方(生半夏+生姜)、生半夏水提物对模型家鸽的止吐作用。 结果: HPLC测定显示小半夏加茯苓汤中姜酚含量较高,以6-姜酚为最高,占23个标记特征峰总峰面积的20%左右。原方及拆方对硫酸铜所致家鸽呕吐均有不同程度抑制作用,止呕强度趋势为原方>拆方>生半夏。 结论: 小半夏加茯苓汤及拆方水提物有一定止吐的作用,结合复方中姜酚含量较高的特征图谱,提示姜酚可能是本方药止吐药效的物质基础之一。

Abstract:

Objective:To establish the characteristic Chart of Xiaobanxia plus Fuling decoction to find the basic substances of its antiemetic effects. Method: HPLC was used to establish the characteristic chart of Xiaobanxia plus Fuling decoction. Fifty pigeons were divided into several groups, treated with salt water or different dosage of Xiaobanxia plus Fuling decoction, Xiaobanxia decoction and Rhizoma Pinelliae. Pigeon vomiting models were estabished by copper sulphate. The latency period of vomiting, vomiting times and frequency were observed to study the antiemetic effect of Xiaobanxia plus Fuling decoction extract and the separating prescription(pinellia temate and ginger, pinellia temate) aqueous extract. Result: The content of ginger phenols in the original decoction was the highest, and among which 6-gingerol was the highest. Both the original decoction and its separating prescription had different degree of antiemetic effect. Xiaobanxia plus Fuling decoction>Xiaobanxia decoction>Rhizoma Pinelliae. Conclusion: Xiaobanxia plus Fuling decoction and its separating decoction have certain antiemetic effect. Considering the chart obtained form HPLC ginger phenols is the major content, which suggests that gingerol may be one of the major basis substance for its antiemetic effect in this prescription.