加味温胆汤对抑郁模型大鼠海马神经细胞内钙离子浓度的影响

Effect of Jiawei Wendan Decoction on Intracellular Calciumion Concentration in Rat Depression Model

摘要:

目的: 探讨加味温胆汤对抑郁模型大鼠海马神经细胞内Ca²⁺浓度干预作用。 方法: 198只SD大鼠随机分为空白组、模型组、中药组、西药组,每组48只。采用孤养结合慢性不可预见性应激造模抑郁模型大鼠,从造模第1天开始中药组给予加味温胆汤12 g·kg^-1·d^-1,西药组予氟西汀1.8 mg·kg^-1·d^-1,模型组、空白组给予生理盐水2 mL·kg^-1·d^-1,各组均灌胃给药,连续28日。在实验第7,14,21,28天,采用激光共聚焦技术检测各组大鼠海马Ca²⁺浓度变化情况。 结果: 在抑郁形成过程中,抑郁大鼠海马Ca²⁺浓度不断上升,21,28 d时,模型组Ca²⁺荧光强度明显高于空白组(P<0.01);经治疗干预,抑郁大鼠海马Ca²⁺荧光强度上升趋势得到抑制,21,28 d时,中、西药组Ca²⁺荧光强度低于模型组(P<0.05或P<0.01)。 结论: 在抑郁形成过程中,海马神经元游离Ca²⁺浓度明显升高,加味温胆汤可能通过抑制海马神经细胞Ca²⁺大量内流,阻止其超载,改善神经可塑性而发挥其抗抑郁效应。

Abstract:

Objective:To probe the intervention effects of Jiawei Wendan decoction on intracellular calciumion concentration of hippocampal neuron in rat depression model. Method: A total of 196 Sptague-Dawley rats were equally and randomly assigned to four group:blank control, model, traditional Chinese medicine treatment group,and western medicine treatment group.Depression model was established,exception for the blank group,by independent feeding and chronic stress for 21 days.In addition,traditional Chinese medicine and western medicine treatment were administered by Jiawei Wendan decoction 12 g·kg^-1·d^-1and fluoxetine 1.8 mg·kg^-1·d^-1,respectively.Model and control group were treated with saline for 28 days. At the 7th, 14th, 21st, 28th day of the experiment, we detected changes of Ca²⁺ concentration in hippocampus neuron of each group by confocal laser technology. Result: During the progress of depression, the concentration of Ca²⁺ in depressive rat's hippocampus became higher and higher. At the 21st and 28th day, fluorescence intensity of Ca²⁺in the model control group was higher than that of the blank group. After administration, this situation had been improved that fluorescence intensity of Ca²⁺in Jiawei Wendan decoction-treated group and fluoxetine-treated group was both lower than that of the model control group at the 21st and 28th day(P<0.05 or P<0.01). Conclusion: During the progress of depression, the concentration of Ca²⁺ increase obviously. Excessive influx of Ca²⁺ in hippocampus neuron can be inhibited by giving Jiawei Wendan decoction as to improve neuroplasticity for producing antidepressive effect.