虚寒状态大鼠免疫系统功能变化研究

Change of Immunologic Function in Deficiency Cold Status

摘要:

目的: 探讨氢化可的松诱导的虚寒大鼠免疫系统功能变化特点,为虚寒证动物模型深入研究奠定基础。 方法: 48只SD雄性大鼠按体重随机分成正常组和虚寒组,每组24只。虚寒组im氢化可的松琥珀酸钠生理盐水溶液20 mg·kg^-1。注射第7,14,21天下午,剪尾取10 μL全血,显微镜下白细胞计数。第15,22天上午,正常组和虚寒组各12只,麻醉,腹主静脉取血,分离血清和血浆(EDTA抗凝),全自动生化分析仪检测免疫球蛋白G(IgG)和免疫球蛋白M(IgM),ELISA法检测白细胞介素4(IL-4)和干扰素-γ(IFN-γ)。摘取胸腺、脾脏电子分析天平称量后,计算脏器指数。 结果: 与同期正常组比较,虚寒组大鼠胸腺指数和血清IgM含量在造模第14,21天均明显降低(P<0.01,P<0.05);脾脏指数和血清IgG含量在造模第14天明显降低(P<0.05),造模第21天未见统计学差异;外周血白细胞数在造模第7,14,21天均明显降低(P<0.01,P<0.05);在造模第21天,血浆IL-4含量降低(P<0.05),IFN-γ含量升高(P<0.05),IFN-γ/IL-4升高(P<0.01)。 结论: 氢化可的松塑造的虚寒状态动物模型,免疫系统功能明显低下,并存在Th1/Th2细胞因子的漂移现象。

Abstract:

Objective:To investigate the change of immunologic function in deficiency cold model induced by hydrocortisone. Method: Forty eight male SD rats were divided into two groups. The control group was intramuscularly injected saline 0.25 mL, the experimental group was intramuscularly injected hydrocortisone sodium succinate saline 20 mg·kg^-1. 10 μL blood sample was collected from tail on 7th day, 14th day and 21th day.WBC count was detected by the microscope. 5 mL blood sample was collected from abdominal main vein on 15th day and 22th day. Serum IgG and IgM levels were detected by automatic biochemical analyzer. Plasma interleukin-4(IL-4) and interferon-γ(IFN-γ) levels were determinated by elisa. Then thymus and spleen were removed and weighed to calculate the organ index. Result: Compared with the control group, the thymus index and serum IgG of experimental group were significantly lower on 14th day and 21th day(P<0.01,P<0.05), the spleen index and serum IgM were significantly lower on 14th day(P<0.05)and no change on 21th day, the WBC count were significantly lower on 7th day, 14th day and 21th day(P<0.01, P<0.05), plasma IL-4 was low(P<0.05), IFN-γ level was high(P<0.05) and IFN-γ/ IL-4 was high(P<0.05)on 21th day. Conclusion: During the experimental process, the immunologic function was significantly lower, and associated with shift of Th1/Th2 in deficiency cold status.