天麻乙酸乙酯提取物对大脑中动脉栓塞模型大鼠神经突触可塑性的影响

Effects of Ethyl Acetate Extraction from Gastrodia elata on Neuroplasticity of Rat Cerebral Ischemia Injury Model

摘要:

目的: 观察天麻乙酸乙酯提取物对大脑中动脉栓塞模型大鼠神经突触可塑性的影响。 方法: 将动物随机分为3组,即假手术组、模型组、天麻乙酸乙酯提取物组(以下简称乙提组,剂量按生药量计为7.29 g·kg^-1)。预防性连续灌胃给予药物7 d,末次给药0.5 h后复制大鼠大脑中动脉栓塞模型,然后再进行治疗性连续灌胃给药7 d,统计模型复制后7 d时的死亡率、各组大鼠在规定时间点进行神经行为学评定及脑组织取材检测GAP-43的表达。 结果: 与假手术组比较,模型组大鼠死亡率和神经病学评分明显升高、肌力评分和GAP-43表达明显降低;与模型组比较,乙提组大鼠术后第7天死亡率和神经病学评分明显降低、肌力评分和GAP-43表达明显升高,差异有统计学意义(P<0.05,P<0.01)。 结论: 天麻乙酸乙酯提取物对大脑中动脉栓塞模型大鼠神经突触可塑性有一定的改善作用。

Abstract:

Objective:To observe the effects of ethyl acetate extraction from Gastrodia elata on the neuroplasticity of rat cerebral ischemia injury model. Method: Sprague-Dawley rats were randomly divided into sham-operation group,model group and ethyl acetate extraction from G. elata group(ethyl acetate extraction group, 7.29 g·kg^-1). The rat models of middle cerebral artery occlusion were replicated 0.5 h later after the last dose of continuous oral administration of drug on the 7th day and keep administration of drug for another 7 days. Statistic data were collected on death rate of model on 7th day,as well as neurological behavior and expression of GAP-43 at specified time point. Result: Compared with the sham-operation group, the death rate and neurological syndromes of model group were markedly increased and the hanging wire and expression of GAP-43 were significantly decreased. Compared with the model group, the death rate and neurological syndromes of the ethyl acetate extraction group were significantly decreased and the hanging wire and expression of GAP-43 were significantly increased (P<0.05,P<0.01). Conclusion: The results suggest that the ethyl acetate extraction from G. elata can improve the neuroplasticity of cerebral ischemia injury model rat.