全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响

Effects of Scorpio-Scolopendra on Expressions of Airway Inflammation and Airway Remodeling in Asthmatic Rats

摘要:

目的: 观察全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响,初探全蝎-蜈蚣治疗哮喘的作用机制。 方法: 30只雄性SD大鼠随机分为3组:正常组(A组)、模型组(B组)、全蝎-蜈蚣组(C组),每组10只。以卵白蛋白致敏并长期吸入激发,制备大鼠支气管哮喘模型。C组予以全蝎-蜈蚣(0.625 g·kg^-1·d^-1)ig,每天1次,A组、B组则同时予以等量生理盐水ig,治疗3周。检测各组大鼠支气管肺泡灌洗液(BALF)中白细胞(WBC)、嗜酸粒细胞(E)、吞噬细胞(M)、淋巴细胞(L)、中性粒细胞(N)计数及百分比,取支气管肺组织制作病理切片,HE染色、Masson染色观察各组大鼠支气管肺组织病理改变,医学图像分析软件检测支气管壁厚度(Wat/Pbm)、平滑肌厚度(Wam/Pbm)、胶原纤维厚度(Wac/Pbm),反映气道重塑的程度。 结果: 与正常组比较,模型组大鼠BALF中细胞数量、中性粒细胞、淋巴细胞、嗜酸粒细胞比例明显增加(P<0.01),模型组Wat/Pbm, Wam/Pbm,Wac/Pbm分别为(155.32±39.92),(22.11±3.42),(8.47±3.01)μm²/μm,正常组分别为(71.01±11.02),(5.51±1.76),(2.37±1.03)μm²/μm,模型组支气管壁、平滑肌层厚度明显增加(P<0.01)、胶原纤维明显增生(P<0.01);与模型组比较,全蝎-蜈蚣组大鼠BALF中细胞数量、中性粒细胞、淋巴细胞、嗜酸粒细胞比例明显减少(P<0.01),Wat/Pbm, Wam/Pbm,Wac/Pbm分别为(100.67±15.04),(17.08±4.92),(4.07±1.08)μm²/μm,支气管壁、平滑肌层厚度明显减轻(P<0.01)、胶原纤维增生程度明显减轻(P<0.01)。 结论: "全蝎-蜈蚣"可改善哮喘模型大鼠气道炎症,同时减少支气管壁和平滑肌厚度,减轻胶原纤维增生,对气道重塑有一定的改善或延缓作用。

Abstract:

Objective:To observe the impact of Scropio-Scolopendra on airway inflammation and airway remodeling of asthmatic rats, and to explore the mechanism of action of Scropio-Scolopendra for asthma. Method: Thirty SD rats were randomly divided into three groups:normal control group(group A),model control group(group B), Scropio-Scolopendra group(group C),10 in each group.The asthmatic rat airway remodeling model was established by ovalbumin sensitized method, The rats in C group was administrated Scropio-Scolopendra by ig(0.625 g·kg^-1·d^-1),and in A,B group rats were administrated the equal physiological saline in the same way. After three weeks of treatment, the number and the percentage of eosinophil(E),macrophage(M),lymphocyte(L),neutrophilic in bronchoalveolar lavage fluid(BALF).Pulmonary pathological changes were observed by HE stain and Masson stain.The thickness of bronchial wall (Wat/Pbm),smooth muscle(Wam/Pbm) and collagen fibers(Wac/Pbm)were determined in medical image analysis software. Result: :Compared with normal group,number of total leukocytes and the percentage of eosinophil,neutrophil,lymphocyte in BALF were significantly higher in the model group(P<0.01). The thickness of bronchial wall (Wat/Pbm), smooth muscel layer(Wam/Pbm) and collagen fibers(Wac/Pbm) in model group [(155.32±39.92),(22.11±3.42),(8.47±3.01) μm²/μm] were all significantly increased than those in normal group [(71.01±11.02),(5.51±1.76),(2.37±1.03) μm²/μm](P<0.01). Compared with model group, number of total leukocytes and the percentage of eosinophil,neutrophil,lymphocyte in BALF were significantly lower in Scropio-Scolopendra group [(100.67±15.04),(17.08±4.92),(4.07±1.08) μm²/μm](P<0.01).The thickness of bronchial wall (Wat/Pbm), smooth muscel layer(Wam/Pbm) and collagen fibers(Wac/Pbm) were significantly decreased in Scropio-Scolopendra group (P<0.01). Conclusion: Scropio-Scolopendra can reduce airway inflammation of asthmatic rats, and play an inhibitory role in airway remodeling by reducing the bronchial wall and smooth muscle thickness, the proliferation of collagen fibers.