黄芩汤对TNBS诱导的结肠炎大鼠IL-23/IL-17通路的影响

Effect of Huangqin Tang on IL-23/IL-17 Pathway in TNBS-induced Colitis

摘要:

目的: 观察黄芩汤对2,4,6-三硝基苯磺酸(TNBS)诱导的结肠炎大鼠白细胞介素23(IL-23)/白细胞介素17(IL-17)通路的影响。 方法: 将32只大鼠随机分为正常对照组、TNBS结肠炎组、TNBS+黄芩汤治疗组(1.2 g·kg^-1)、TNBS+美沙拉嗪治疗组(10 mL·kg^-1 ,ig),每组8只。除正常对照组外,其余各组均应用2,4,6-三硝基苯磺酸(TNBS)灌肠法建立结肠炎模型,造模完成后,各组分别按设计剂量连续给药1周。用酶联免疫吸附法(ELISA)分析大鼠血清中白细胞介素17(IL-17)、白细胞介素23(IL-23)的水平,用real-time PCR方法检测大鼠结肠组织中IL-17,IL-17R,IL-23,IL-23R,RORγt的mRNA水平。 结果: 黄芩汤治疗能有效降低TNBS诱导的结肠炎大鼠血清中IL-17(262.75±17.19 ng·L^-1和IL-23(56.75±6.20) ng·L^-1的蛋白水平,降低结肠组织中IL-17(2.606±0.8),IL-17R(5.33±1.10),IL-23(2.16±0.19),IL-23R(3.34±0.70),RORγt(0.74±0.17)的mRNA水平。 结论: 黄芩汤能有效的调节TNBS诱导的结肠炎大鼠IL-23/IL-17通路,这可能是其治疗结肠炎的免疫机制之一。

Abstract:

Objective:To evaluate the effects of Huangqin Tang on the IL-23/IL-17 pathway in 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis model. Method: SD rats were distributed into four group, containing TNBS-induced colitis,Huangqin Tang(1.2 g·kg^-1) or mesalazine treatment group(10 mL·kg^-1,ig),negative control with no treatment and the last one was normal sham rats. Except for control group,experimental colitis was induced by rectal instillation of 2,4,6-trinitrobenzene sulfonic acid (TNBS) into rats. Drugs were given intragastrically for one week in each group continuously according to respectively-designed doses. The protein expressions of IL-17 and IL-23 in serum were determined by ELISA. The mRNA expressions of IL-17, IL-23, IL-17R, IL-23R and RORγt were determined by Real-time PCR. Result: Huangqin Tang markedly attenuated TNBS-induced colitis and effectively inhibited the protein expressions of IL-17(262.75±17.19) ng·L^-1 and IL-23(56.75±6.20) ng·L^-1. Furthermore, mRNA levels of IL-17(2.606±0.8),IL-17R(5.33±1.10),IL-23(2.16±0.19),IL-23R(3.34±0.70),RORγt(0.74±0.17)showed a marked reduction after Huangqin Tang teatment. Conclusion: Taken together, our findings demonstrate that Huangqin Tang can exerte therapeutic effects on TNBS-induced colitis, mediate probably by the IL-23/IL-17 pathway.