玉郎伞多糖对慢性应激抑郁小鼠行为学及海马结构的影响

Effects of Yulangsan Polysaccharides on Behavior, Structure of Hippocampus in Mice with Chronic Stress-induced Depression

摘要:

目的: 观察玉郎伞多糖(YLSPA)对慢性应激抑郁小鼠行为学及海马结构的影响。 方法: 50只昆明种小鼠随机分为5组:正常对照组、模型组、氟西汀20 mg·kg^-1 组和YLSPA 1 200,600 mg·kg^-1 高、低剂量组,连续灌胃21 d,同期利用慢性不可预知温和应激加孤养模型建立小鼠抑郁症模型。采用蔗糖水消耗实验和小鼠自主活动记录仪进行行为学测定;采用脑组织切片HE染色观察海马结构的改变。 结果: 实验第22天,模型组小鼠的体重、活动站立次数、糖水偏爱度明显减少(与正常对照组比较,P<0.01或P<0.05),YLSPA高、低剂量组、氟西汀组均较模型组有所增加(P<0.01或P<0.05);观察HE染色切片可见慢性应激引起海马结构发生改变,细胞数量减少,玉郎伞多糖和氟西汀可对抗这种改变。 结论: YLSPA具有抗抑郁作用,能改善慢性应激对小鼠海马神经元细胞的损伤。

Abstract:

Objective:To observe the effects of Yulangsan polysaccharides (YLSPA) on behavior, structure changes of hippocampus in mice with chronic stress-induced depression. Method: Fifty Kunming mice were randomly divided into 5 groups: normal control, animal model, fluoxetine 20 mg·kg^-1 and YLSPA 1 200 mg·kg^-1, YLSPA 600 mg·kg^-1 groups with continuous oral administration for 21 d, and chronic unpredictable mild stress (CUMS) with isolated support was imposed simultaneously to establish mouse depression model. The behavior changes of mice were evaluated by sucrose water consumption and the spontaneous activity; the structure changes of hippocampus were detected by the brain tissue HE staining. Result: The weight, the number of active stand, the consumption and preference for sucrose solution of the animal model group were significantly decreased compared with normal control group (P<0.01 or P<0.05), and were increased in YLSPA group and fluoxetine group (P<0.01 or P<0.05). Chronic unpredictable mild stress with isolated support could damage the structure of hippocampus, reduce the number of cells, YLSPA and fluoxetine could ameliorate these changes. Conclusion: YLSPA can improve the behavior scores of the mice with depression and ameliorate the damage of hippocamus induced by chronic unpredicted mild stress.