兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠免疫调控机制的影响

Effect of Lanyin Fengyang Huazhuo Jiedu Prescription on Immune Regulation Mechanism in Rats with Ulcerative Colitis

摘要:

目的: 观察兰茵凤扬化浊解毒方对溃疡性结肠炎(UC)大鼠CD4-T淋巴细胞(CD4⁺)/CD29-T淋巴细胞(CD29⁺)、白细胞介素-8(IL-8)及白细胞介素-10(IL-10)表达的影响,以探讨其作用机制。 方法: 将60只雄性Wistar大鼠随机分为5组,每组12只,分别为正常组、模型组、兰茵凤扬化浊解毒方高剂量组、低剂量组(20.0, 5.0 g·kg^-1)、柳氮磺胺吡啶对照组(SPAP 0.3 g·kg^-1组)。采用2,4,6-三硝基苯磺酸(TNBS)/乙醇法诱导溃疡性结肠炎模型,造模成功后分别ig给予相应的药物,连续14 d,观察各组大鼠一般情况以及疾病活动指数(DAI)评分、组织病理学、CD4⁺CD29⁺,IL-8,IL-10变化情况。 结果: DAI评分:与正常组比较,其余各组均升高;与模型组比较,兰茵凤扬高、低剂量组均减低,结果均有统计学意义(P<0.05)。CD4⁺,CD29⁺:与模型组相比,对照组、中药高、低剂量组均降低,结果有统计学意义(P<0.05)。IL-8: 与模型组相比,对照组、中药高、低剂量组均减低(P<0.05),而中药低剂量组与对照组比较无差异。IL-10: 与模型组相比,中药高、低剂量组、对照组均升高(P<0.05)。 结论: 兰茵凤扬化浊解毒方可上调抑炎症因子、下调促炎症因子和抑制CD4⁺/CD29⁺的表达。

Abstract:

Objective:To observe the effect of Lanyin Fengyang Huazhuo Jiedu prescription on expressions of CD4⁺/CD29⁺,IL-8 and IL-10 in rats with ulcerative colitis. Method: Sixty healthy male Wistar rats were randomly divided into 5 groups: the normal group(NG), model control group (MG), high-dose group of Lanyin Fengyang Huazhuo Jiedu decoction (HDG,20.0 g·kg^-1), low-dose group of Lanyin Fengyang Huazhuo Jiedu decoction (LDG,5.0 g·kg^-1) and the sulfasalazine group(SPAP 0.3 g·kg^-1),12 rats in every group. Trinitrobenzenesu l-fonicacid/ethanol(TNBs/ethanol) was used to induce ulceratvie colitis. After successful modeling, continuous treatment lasted 14 days. The rat's general state, disease activity index(DAI) scores,colonic pathological morphology change of T-lymphocyte cytokines IL-8 and IL-10 were observed. Result: DAI scores: compared with NG,the scores of other groups were elevated. compared with the MG, the scores of HDG and LDG were reduced. The result showed statistical significance (P<0.05). CD4⁺,CD29⁺: compared with the MG, the levels of NG,HDG and LDG were reduced. The result showed statistical significance (P<0.05). IL-8: compared with the MG, the levels of NG,HDG and LDG were reduced (P<0.05). There was no difference between the levels of LDG and SG. IL-10: compared with the MG, the levels of SPAP,HDG and LDG were elevated(P<0.05). Conclusion: Lanyin Fengyang Huazhuo Jiedu decoction can raise the suppression of inflammatory cytokines,reduce promoting the inflammatory factor and suppressioned of CD4⁺/CD29⁺expression