绞股蓝多糖对四氯化碳所致大鼠肝损伤的保护作用

Protective Effect of Gynostemma pentaphyllum Polysaccharide on Liver Injure Induced by Carbon Tetrachloride in Rats

摘要:

目的: 研究绞股蓝多糖对四氯化碳诱导大鼠肝损伤的保护作用。 方法: 大鼠以50% CCl₄ 花生油溶液按1 mL·kg^-1 ig,连续4周建立实验性肝损伤模型。随机分为4组:模型对照组、联苯双酯组、绞股蓝多糖低、高剂量组,并设正常对照组。ig绞股蓝多糖40,80 g·kg^-1,联苯双酯40 mg·kg^-1,正常对照组及模型组ig给予等量生理盐水,连续ig 30 d。末次给药24 h后取材,检测大鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的含量,逆转录聚合酶链式反应(RT-PCR)法测定肝组织中诱导型一氧化氮合酶 (iNOS) mRNA的表达,Western blot法测定肝组织中B细胞淋巴瘤/白血病-2基因(Bcl-2)/Bcl-2相关X蛋白(Bax)凋亡相关蛋白的表达,并观察肝组织病理学变化。 结果: 与模型组比较,绞股蓝多糖低、高剂量给药组有效降低肝损伤大鼠血清中AST(85.28±4.09,72.49±3.62)U·L^-1,ALT(75.31±3.95),(58.26±4.83)U·L^-1水平(P<0.05),下调组织中iNOS mRNA的表达(P<0.05)。治疗后大鼠肝组织中Bcl-2/Bax蛋白水平均有所升高(P<0.05),并减轻肝损伤病情。 结论: 绞股蓝多糖对CCl₄诱导的大鼠肝损伤具有一定保护作用,其机制可能与其抑制细胞毒作用以及抗凋亡途径有关。

Abstract:

Objective:To study the protective effect of the Gynostemma pentaphyllum polysaccharide on the CCl₄-induced liver injure in rats. Method: The liver injure was induced by carbon tetrachloride(CCl₄) in rats, rats were divided into four groups randomly: model group, Bifendate group (40 mg·kg^-1), low-and high-dose groups of G. pentaphyllum polysaccharide (40, 80 g·kg^-1), as well as normal group was set up for 30 consecutive days. At the end of 30 days, the blood was collected and the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were examined, the expression of inducible nitric oxide synthase(iNOS) mRNA in liver were examined by reverse transcription polymerase chain reaction(RT-PCR) analysis. The expression of B cell lymphoma/leukmia-2(Bcl-2)/Bcl-2 associated X protein(Bax) in hepatic tissue was determined by Western blot analysis, and the changes of hepatic histopathology was observed by HE-staining. Result: Compared with model control group, G. pentaphyllum polysaccharide significantly decreased the levels of AST and ALT in liver injure induced by CCl₄in rats (P<0.05), while the iNOS mRNA expression in hepatic tissue was down-regulated (P<0.05). The level of Bcl-2/Bax was elevated in hepatic tissue (P<0.05), and liver injure was alleviated. Conclusion: The results disclose that G. pentaphyllum polysaccharide has protective effect on the CCl₄-induced liver injure in rats, mechanism of which may be related to inhibiting the cytotoxicity and anti-apoptotic pathways.