附子理中汤对脾阳虚大鼠骨骼肌能荷及CNTF mRNA的影响

Influence of Energy Charge and Expression of CNTF mRNA in Rats with Spleen Yang Deficiency Syndrome after Administrated Fuzi Lizhong Decoction

摘要:

目的: 观察附子理中汤对脾阳虚大鼠骨骼肌能荷及睫状神经营养因子(CNTF)mRNA表达的影响。 方法: 大鼠分为对照组、模型组和附子理中汤组,模型组施行肩胛骨间棕色脂肪组织(brown adipose tissue,BAT)切除术,饲以高脂饲料,19℃环境喂养3周。附子理中汤组在模型组基础上以附子理中汤按5 mL·kg^-1体重ig,每日1次,连续ig4周。测定大鼠骨骼肌能荷值,RT-PCR 法检测CNTF mRNA表达。 结果: 与对照组大鼠骨骼肌能荷(0.042±0.018)比较,模型组(0.032±0.014)与附子理中汤组(0.024±0.012)的能荷值均降低(P<0.05或P<0.01)。与模型组比较,附子理中汤组的能荷值降低明显(P<0.05)。对照组CNTF mRNA相对表达量为(0.862±0.072),模型组CNTF mRNA相对表达量(0.426±0.056)较对照组降低(P<0.01)。与模型组比较,附子理中汤组CNTF mRNA相对表达量(0.838±0.068)升高(P<0.01)。 结论: 附子理中汤对CNTF mRNA表达有调节作用,通过上调CNTF mRNA表达,增加CNTF含量,发挥CNTF对骨骼肌营养作用和能量代谢调节作用。

Abstract:

Objective:To observe the influence of energy charge and the expression of ciliary neurotrophic factor mRNA(CNTF mRNA) by Fuzi Lizhong decoction in rats with spleen yang deficiency syndrome. Method: Animals were divided into the control goup,the model group and the Fuzi Lizhong decoction group. The model group was insised brown adipose tissue then fed with high fat diet under 19℃ circumstance,which lasted for 3 weeks.The Fuzi Lizhong decoction group was ig 5 mL·kg^-1 Fuzi Lizhong decoction based on the model group once a day, for 4 weeks. Testing energy charge of skeletal muscle and the expression of CNTF mRNA were assayed by the method of reverse transcription-polymerase chain reaction(RT-PCR). Result: Compared with the control group(0.042±0.018), energy charge decreased in the model group (0.032±0.014)and the Fuzi Lizhong decoction group (0.024±0.012 )( P<0.05 or P<0.01). Compared with the model group, energy charge decreased significantly (P<0.05). Compared with the control group(0.862±0.072),the expression of CNTF mRNA in the model group (0.426±0.056) decreased(P<0.01). Compared with the model group, the expression of CNTF mRNA in the Fuzi Lizhong decoction group (0.838±0.068)increased (P<0.01). Conclusion: It is suggested that Fuzi Lizhong decoction can regulate the expression of CNTF mRNA, which indicated an effect on nutrition and energy metabolism of skeletal muscle by up-regulating the expression of CNTF mRNA and increasing the content of CNTF.