注射用三七素对SD大鼠神经系统的影响

Effect of Dencichine Injection on Nervous System of SD Rats

摘要:

目的: 研究注射用三七素对SD大鼠神经系统的影响。 方法: 大鼠尾静脉给药,每日1次,连续7 d。将SD大鼠随机分成4组,空白对照组、三七素低、中、高剂量组(15,45,135 mg·kg^-1),于给药后1,2 h,7 d及停药后考察注射用三七素对自发活动的影响;将SD大鼠随机分成5组,空白对照组、三七素低、中、高剂量组(15,45,135 mg·kg^-1)、阳性对照组(7.5 mg·kg^-1戊巴比妥钠),于给药后1 h,7 d及停药后来考察注射用三七素对运动协调能力的影响。 结果: 注射用三七素在135 mg·kg^-1剂量下会抑制SD大鼠的自发活动和运动协调能力,该抑制作用停药后即可缓解并消除;在45 mg·kg^-1剂量下单次给药后SD大鼠的自发活动有所增加,停药后消除,对运动协调能力未见明显影响;在15 mg·kg^-1剂量下,SD大鼠的自发活动在停药后有所增加,但对运动协调能力无明显影响。 结论: 注射用三七素在135 mg·kg^-1 剂量下对SD大鼠的神经系统有明显抑制作用;在45 mg·kg^-1 剂量下对神经系统有短时兴奋作用;在15 mg·kg^-1剂量下对SD大鼠的神经系统无明显影响。

Abstract:

Objective:To study the effect of dencichine injection on nervous system. Method: The rats were given drug by iv once daily for 7 consecutive days. The SD rats were divided into 4 groups randomly: normal saline(NS)group, the low dose group, middle dose group and high dose group, the effect of dencichine for injection on autonomic activities was observed after administration of 1, 2 h,7 d and withdrawal of injection. The SD rats were divided into 5 groups randomly: normal saline (NS) group, the low dose group, middle dose group, high dose group and positive control group(7.5 mg·kg^-1 Pentobarbital sodium), the effect of dencichine for injection on concordant ability was observed after administration of 1 h, 7 d and withdrawal of injection. Result: Dencichine injection at the dose of 135 mg·kg^-1 reduced autonomic and concordant ability of rats,this phenomenon disappeared after stopping injecting drugs. Dencichine injection at the dose of 45 mg·kg^-1 increased autonomic ability of rats, but the phenomenon disappeared soon, and the dose of 45 mg·kg^-1 had no effect on the concordant ability. Dencichine for injection at the dose of 15 mg·kg^-1 increase autonomic ability of rats in a short time after stopping injecting drugs,but it had no effect on the concordant ability. Conclusion: Dencichine injection at the dose of 135 mg·kg^-1 has obvious effect on nervous system. Dencichine injection at the dose of 45 mg·kg^-1 has excitatory effects on the nervous system in a short period of time. Dencichine for injection at the dose of 15 mg·kg^-1 have no effect on the nervous system, which can be believed a safety dose.