牛心柿叶多糖对糖尿病小鼠肾脏损伤的保护作用

Protective Effect of the Niuxin Persimmon Leaf Polysaccharide on Kidney Injure in Diabetic Mice

摘要:

目的: 探讨牛心柿叶多糖对链脲佐菌素致糖尿病小鼠肾脏损伤的保护作用及其机制。 方法: 建立糖尿病小鼠模型,随机分成5组:模型组、二甲双胍阳性组及牛心柿叶多糖低、中、高剂量组。ig给予牛心柿叶多糖 0.3, 0.6, 1.2 g·kg^-1,阳性组给予二甲双胍0.32 g·kg^-1,连续ig 15 d。末次给药后,氧化酶法测定空腹血糖(FBG),终点法测24 h尿微量白蛋白含量。 苏木精-伊红染色法(HE染色)观察肾脏形态学变化。蛋白质印迹(Western blot)法检测肾脏组织中胰岛素样生长因子 (IGF-1) 表达。 结果: 与模型对照组比较,牛心柿叶多糖给药组中糖尿病小鼠的血糖明显降低(P<0.05),并显著降低尿微量白蛋白含量(P<0.05)。下调肾脏组织中IGF-1蛋白水平 (P<0.05),并缓解肾损伤程度。 结论: 牛心柿叶多糖对链脲佐菌素所致糖尿病小鼠肾脏损害具有保护作用,机制可能与其通过抑制肾脏组织中IGF-1的表达而调节其下游通路有关。

Abstract:

Objective:To study the protective effect of the Niuxin persimmon leaf polysaccharide on kidney injure in diabetic mice induced by streptozotocin(STZ) and its mechanisms. Method: Being established of diabetic mice model, the mice were randomly assigned to 5 groups: model group, melbinum group (0.32 g·kg^-1), low, medium and high-dose groups of Niuxin persimmon leaf polysaccharide (0.3, 0.6, 1.2 g·kg^-1) for 15 consecutive days. At the end of 15 days, oxidase method was used to examine the blood glucose levels (fasting blood-glucose,FBG), while the endpoint method was used to measured urinary albumin content (24 h). The changes in renal morphology were tested through HE-staining. The expressions of insulin-like growth factors-1(IGF-1) in renal tissue were determined using Western blot analysis. Result: Compared to model control group, Niuxin persimmon leaf polysaccharide could notably diminish the FBG in diabetic mice (P<0.05), and measured urinary albumin content was reduced statistically (P<0.05). The IGF-1 protein level in renal tissue was up-regulated (P<0.05), and the condition of renal injury alleviated finally. Conclusion: The results indicate that Niuxin persimmon leaf polysaccharide has protective effect on renal injury in diabetic mice induced by STZ. The mechanism may be related to inhibition of IGF-1 expression in renal tissue to regulate its downstream pathway.